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Updated: Jan 20, 2026

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
Published on: August 21, 2014
Northern Blot with IR Fluorescent Probes: Strategies for Probe Preparation
Christopher Fields1,2, Peike Sheng1,2, Bret Miller1
1Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, United States.
Abstract:
Northern blot is a molecular biology technique that can detect, quantify, and determine the molecular weight of RNA. Recently, we published a protocol utilizing near-infrared (IR) fluorescent probes in Northern blot (irNorthern). Our method is as sensitive as other non-radioactive methods but is more straightforward and versatile. Additionally, we found that IR-labeled probes can be used to multiplex or detect different species of RNA at the same time. Here we describe three methods for generating an IR-labeled probe as well as how to perform irNorthern blot. In conclusion, our irNorthern protocol offers a convenient method for RNA detection.
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