Graphene oxide composites for magnetic solid-phase extraction of twelve quinolones in water samples followed by...

Antibiotic compounds in natural waters are normally present at low concentrations. In this paper, an easy and highly sensitive screening method using graphene oxide-functionalized magnetic composites (GO@NH₂@Fe₃O₄) combined with matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) was established for twelve quinolone antibiotics. GO@NH₂@Fe₃O₄ composites were utilized as adsorbents for magnetic solid-phase extraction. This method combines the advantages of magnetic solid-phase extraction and MALDI-TOF MS, which allows for fast detection of quinolones at low concentrations. To improve absorption efficiency, the following parameters were individually optimized: sample acidity, extraction time, amount of adsorbent used, eluent used, and desorption time. Under the optimum conditions, the established method gave a low detection limit of 0.010 mg/L and allowed the high-throughput screening of twelve quinolone antibiotics (enoxacin, norfloxacin, ciprofloxacin, pefloxacin, fleroxacin, gatifloxacin, enrofloxacin, levofloxacin, sparfloxacin, danofloxacin, difloxacin, and lomefloxacin). The proposed method, having an easily prepared sorbent with a high affinity for quinolones and a convenient, high-throughput detection step, has been shown to have merit for the detection of antibiotics in water samples. Graphical abstract Schematic illustration of the (A) preparation of GO@NH₂@Fe₃O₄ and (B) operating procedure for the MSPE and MALDI-TOF MS detection of QNs.