Urinary markers of hydration during 3-day water restriction and graded rehydration

This investigation had three purposes: (a) to evaluate changes in hydration biomarkers in response to a graded rehydration intervention (GRHI) following 3 days of water restriction (WR), (b) assess within-day variation in urine concentrations, and (c) quantify the volume of fluid needed to return to euhydration as demonstrated by change in U_col.

115 adult males and females were observed during 1 week of habitual fluid intake, 3 days of fluid restriction (1000 mL day^-1), and a fourth day in which the sample was randomized into five different GRHI groups: no additional water, CON; additional 500 mL, G_+0.50; additional 1000 mL, G_+1.00; additional 1500 mL, G_+1.50; additional 2250 mL, G_+2.25. All urine was collected on 1 day of the baseline week, during the final 2 days of the WR, and during the day of GRHI, and evaluated for urine osmolality, color, and specific gravity.

Following the GRHI, only G_+1.50 and G_+2.25 resulted in all urinary values being significantly different from CON. The mean volume of water increase was significantly greater for those whose U_col changed from > 4 to < 4 (+ 1435 ± 812 mL) than those whose U_col remained ≥ 4 (+ 667 ± 722 mL, p < 0.001).

An additional 500 mL of water is not sufficient, while approximately 1500 mL of additional water (for a total intake between 2990 and 3515 mL day^-1) is required to return to a urine color associated with adequate water intake, following 3 days of WR.