Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

537
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
537
What is Gene Expression?01:42

What is Gene Expression?

194.7K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
194.7K
Constitutive and Regulated Gene Expression01:27

Constitutive and Regulated Gene Expression

961
Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
961
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

25.7K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
25.7K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

16.3K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.3K
Chromatin Position Affects Gene Expression02:35

Chromatin Position Affects Gene Expression

24.6K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences  access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area. 
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Dual-gRNA CRISPR/Cas9 System for Efficient Generation of Large Fragment Deletions in Poplar.

Bio-protocol·2026
Same author

Kit Ligand enhances in vitro oocyte maturation and early embryonic development in sheep.

Animal reproduction science·2026
Same author

A Novel Sperm Swimming up Trapper Method Enhances Sperm Quality and ART Outcomes by Avoiding Centrifugation-Induced Damage in a Murine Model†.

Biology of reproduction·2026
Same author

From Defense Executor to Engineering Target: Harnessing Lignin for Crop Resistance.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026
Same author

RETRACTED: Impact of evidence-based nursing on surgical site wound infection after caesarean: A meta-analysis.

International wound journal·2026
Same author

Practical Integration of Bedside Real-Time Lung Ultrasound and Electrical Impedance Tomography for Guiding Personalized Perioperative Lung Protective Ventilation in Cardiothoracic and Vascular Anesthesia.

Journal of cardiothoracic and vascular anesthesia·2026

Related Experiment Video

Updated: Jan 20, 2026

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

16.0K

Quorum-sensing based small RNA regulation for dynamic and tuneable gene expression.

Shao-Heng Bao1, Wen-Ying Li1, Chang-Jun Liu2

  • 1Research Center of Biological Information, College of Arts and Sciences, National University of Defense Technology, Changsha, Hunan, People's Republic of China.

Biotechnology Letters
|August 21, 2019
PubMed
Summary

Researchers engineered bacterial small RNA to create a pathway-independent strategy for dynamic gene repression. This method allows precise control over gene expression at the post-transcriptional level, enabling fine-tuning of metabolic flux without impacting cell growth.

Keywords:
Bacterial small RNADynamic regulationPathway independentQuorum sensingTuneable regulation

More Related Videos

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

5.7K
Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms
03:29

Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms

Published on: May 31, 2024

899

Related Experiment Videos

Last Updated: Jan 20, 2026

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells
10:34

Using an Automated Cell Counter to Simplify Gene Expression Studies: siRNA Knockdown of IL-4 Dependent Gene Expression in Namalwa Cells

Published on: April 14, 2010

16.0K
Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation
12:26

Optical Tweezers to Study RNA-Protein Interactions in Translation Regulation

Published on: February 12, 2022

5.7K
Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms
03:29

Author Spotlight: Advancing Therapeutics to Treat Vibriosis in Humans and Aquatic Organisms

Published on: May 31, 2024

899

Area of Science:

  • Synthetic Biology
  • Molecular Biology
  • Metabolic Engineering

Background:

  • Developing dynamic regulation strategies is crucial for automatic control systems that manipulate metabolic fluxes and cellular behaviors.
  • A general system for controlling any gene of interest is needed to broaden the application scope of such strategies.

Purpose of the Study:

  • To engineer a pathway-independent strategy for dynamic gene repression at the post-transcriptional level.
  • To develop a system capable of controlling any gene of interest for metabolic flux manipulation.

Main Methods:

  • Engineered bacterial small RNA molecules.
  • Developed a dynamic repression strategy targeting gene expression post-transcriptionally.
  • Utilized modifications in the Plux promoter's lux box for fine-tuning repression levels.

Main Results:

  • The strategy achieved incremental repression of gene expression, ranging from 0% to 90% during culturing.
  • A single base pair change in the lux box of the Plux promoter allowed tuning of target gene repression by 70%.
  • The developed strategy is expected to enable metabolic flux control without adverse effects on cell growth.

Conclusions:

  • A novel bacterial small RNA-based strategy was successfully engineered for pathway-independent, dynamic gene repression.
  • The strategy operates at the post-transcriptional level, offering precise control over gene expression.
  • This approach provides a versatile tool for manipulating metabolic flux and cellular behaviors without disrupting cell growth.