Related Experiment Video
Updated: Jan 20, 2026
08:30
Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
Published on: May 10, 2022
2.5K
Compartmentalized effects of aging on group 2 innate lymphoid cell development and function
Shanti S D'Souza1, Xiaofei Shen1, Ivan T H Fung1
1Department of Immunology and Microbial Disease, Albany Medical College, Albany, New York, USA.
Aging Cell
|August 21, 2019
Summary
Aging impairs lung group 2 innate lymphoid cells (ILC2), compromising immune responses. Restoring these cells in aged mice improved resistance to influenza infection, suggesting therapeutic potential.
Area of Science:
- Immunology
- Aging Research
- Innate Immunity
Background:
- Immunosenescence, the aging of the immune system, has complex effects on innate immunity.
- Group 2 innate lymphoid cells (ILC2) are crucial for lung homeostasis and repair, but their aging dynamics are unclear.
Purpose of the Study:
- To investigate the impact of aging on the development, function, and distribution of ILC2.
- To determine the role of aged ILC2 in pulmonary immune responses and susceptibility to infection.
Main Methods:
- Analysis of ILC2 development and function in young versus aged mice.
- Transcriptomic profiling of aged lung ILC2.
- Functional assays assessing cytokine production and response to influenza infection.
- Adoptive transfer experiments using young ILC2 into aged recipients.
Main Results:
- Aging promotes early ILC2 development in bone marrow but impairs their migration to the lungs.
- Aged lung ILC2 are functionally compromised, exhibiting reduced cytokine production and lower Cyp2e1 expression.
- Transfer of young ILC2 restored resistance to influenza infection in aged mice.
Conclusions:
- Aging causes compartmentalized defects in ILC2, affecting their development, localization, and function.
- Targeting tissue-resident ILC2 may offer therapeutic strategies to combat infections and diseases in the elderly.
Related Concept Videos
01:18Lymphoid Cells and Tissues
3.0K
Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes.
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
3.0K
01:27Pharmacodynamics in Geriatric Patients: Effects of Age
191
Age-related pharmacokinetic changes are extensively documented, but understanding age-related pharmacodynamic alterations is relatively limited. This knowledge gap can be partly attributed to the complexity of developing appropriate measures of drug responses compared to bioanalytical methods for determining drug concentrations.Most information regarding age-related differences in human pharmacodynamics originates from cross-sectional studies. However, these studies assume that observed mean...
191
01:37Eukaryotic Compartmentalization
17.8K
One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal...
For example, lysosomes in the animal...
17.8K
01:58Fruit Development, Structure, and Function
25.0K
Fruits form from a mature flower ovary. As seeds develop from the ovules contained within, the ovary wall undergoes a series of complex changes to form fruit. In some fruits, such as soybeans, the ovary wall dries; in other fruits, such as grapes, it remains fleshy. In some cases, organs other than the ovary contribute to fruit formation; such fruits are called accessory fruits.
25.0K
01:46Eukaryotic Compartmentalizations
172.5K
One of the distinguishing features of eukaryotic cells is that they contain membrane-bound organelles, such as the nucleus and mitochondria, that carry out specialized functions. Since biological membranes are only selectively permeable to solutes, they help create a compartment with controlled conditions inside an organelle. These microenvironments are tailored to the organelle's specific functions and help isolate them from the surrounding cytosol.
For example, lysosomes in the animal cells...
For example, lysosomes in the animal cells...
172.5K
01:28Cells of the Innate Immune Response
8.7K
The innate immune response is an immediate and non-specific response against pathogens, acting swiftly to prevent the spread of infections. The primary cells involved in this response are phagocytes and natural killer (NK) cells.
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
Phagocytes
Phagocytes police the peripheral tissues by removing cellular debris and responding to the invasion of foreign substances or pathogens. Many phagocytes attack and remove microorganisms even before lymphocytes detect them. The human body has two general...
8.7K