Related Experiment Video
Updated: Jan 20, 2026

Plant Promoter Analysis: Identification and Characterization of Root Nodule Specific Promoter in the Common Bean
Published on: December 23, 2017
Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean
Wendell Jacinto Pereira1,2, Arthur Tavares de Oliveira Melo3, Alexandre Siqueira Guedes Coelho3
1Universidade Federal de Goiás, Instituto de Ciências Biológicas, Goiânia, GO, Brazil.
Abstract:
Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans.
Related Concept Videos
Responses to Drought and Flooding
Responses to Salt Stress
Responses to Heat and Cold Stress
Stress Response System
Alarm stage
In the alarm stage, the body's...
Genome-wide Association Studies-GWAS
GWAS does not require the identification of the target gene involved in...
Psychological Responses to Stress

