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Updated: Jan 20, 2026

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
Published on: June 3, 2018
A photoelectrochemical biosensor based on fullerene with methylene blue as a sensitizer for ultrasensitive DNA
Dan Long1, Mengjie Li1, Haihua Wang1
1Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, 400715, PR China.
Abstract:
In this protocol, a photoelectrochemical (PEC) biosensor based on methylene blue (MB) sensitized fullerene (C60) was designed for ultrasensitive DNA detection. First, C60 nanoparticles (C60 NPs) as photoactive material was modified on the electrode surface to obtain an initial photocurrent signal. Additionally, the nicking endonuclease (Nb.BvCI enzyme)-assisted signal amplification strategy was implemented to transform limited target to plenty of products, which hybridized with DNA2 to form plentiful DNA duplex for immobilizing numerous MB as the sensitizer, thus accomplishing the effective sensitization toward C60 NPs and achieving an enhanced photocurrent for quantitatively detecting target DNA. This established PEC strategy realized the target DNA detection with the detection limit down to 3.3 fM, which paved a new avenue for DNA determination and exhibited huge application potential for other biomarkers in clinical analysis and disease diagnosis.
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