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Updated: Jan 20, 2026

Evaluation of Integrated Anaerobic Digestion and Hydrothermal Carbonization for Bioenergy Production
Published on: June 15, 2014
Effect of one step temperature increment from mesophilic to thermophilic anaerobic digestion on the linked pattern
Zuopeng Lv1, Xiayuan Wu2, Bingqian Zhou1
1The Key Laboratory of Biotechnology for Medicinal Plants of Jiangsu Province, School of Life, Jiangsu Normal University, Shanghai Road 101, 221116 Xuzhou, China.
Abstract:
Process fluctuation caused by temperature modification of anaerobic digestion is routinely monitored via operational parameters, such as pH and gas production, but these parameters are lagging on microbial community performance. In this study, 13C isotope fractionation in CH4 and CO2 of biogas together with microbial community dynamics were applied to evaluate process stability in response to temperature increment. Results showed that the weakening correlated links between Firmicutes affiliated families and Methanomicrobiaceae were found regarding temperature increase. In contrast, Methanosarcinaceae and Methanobacteriaceae strengthened their links with multiple bacterial groups. This suggests that the 13C isotope fractionation in CH4 can predict the collapse of certain microbial interconnections and process instability, the new reinforced microbial links directly reflect the microbial community redundancy for maintaining function of syntrophic populations.
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