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Recent advances in microfluidic methods in cancer liquid biopsy
Florina S Iliescu1, Daniel P Poenar2, Fang Yu3
1School of Applied Science, Republic Polytechnic, Singapore 738964, Singapore.
Biomicrofluidics
|August 22, 2019
Summary
On-chip microfluidic devices advance cancer liquid biopsies for early detection and monitoring. Research highlights progress in analyzing circulating tumor cells and other biomarkers, with potential for urine-based diagnostics.
Area of Science:
- Oncology
- Biotechnology
- Microfluidics
Background:
- Early cancer detection, monitoring, and therapeutic prediction are critical but challenging in oncology.
- Liquid biopsies offer a minimally invasive approach to analyze cancer biomarkers in body fluids.
- Recent technological advancements enable the detection and interpretation of cancer biomarkers.
Purpose of the Study:
- To provide an overview of on-chip technological developments for cancer liquid biopsy.
- To discuss current challenges and clinical applications of microfluidic-based liquid biopsies.
- To analyze microfluidic isolation of circulating tumor cells (CTCs) and other biomarkers.
Main Methods:
- Review of recent on-chip microfluidic technologies for cancer liquid biopsy.
- Comparison of microfluidic solutions for various biomarkers (CTCs, nucleic acids, proteins, exosomes, metabolites).
- Detailed analysis of microfluidic strategies for CTC isolation, purity, and capture efficiency.
Main Results:
- Significant progress in microfluidic devices for detecting and monitoring cancer biomarkers.
- Circulating tumor cells (CTCs) are a key focus, but other biomarkers (proteins, exosomes, cfNA) also provide clinical insights.
- Urine presents an underexplored but promising source for molecular cancer biomarkers in point-of-care devices.
Conclusions:
- On-chip microfluidic technologies are advancing cancer liquid biopsy capabilities.
- Challenges remain in clinical translation and application, but trends indicate future potential.
- Future research should explore alternative biomarkers and matrices like urine for accessible diagnostics.
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