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Differential expression of circRNAs during rat lung development
Yan-Qing Shen1, Jing-Jing Pan2, Zhong-Yi Sun2
1Department of Neonates, Children's Hospital of Nanjing Medical University, Nanjing, Jiangsu 210008, P.R. China.
International Journal of Molecular Medicine
|August 22, 2019
Summary
This study identifies novel circular RNAs (circRNAs) crucial for rat lung development. These findings enhance understanding of lung development and related diseases.
Area of Science:
- Developmental Biology
- Genomics
- Molecular Biology
Background:
- Circular RNAs (circRNAs) are increasingly recognized for their roles in various biological processes.
- The expression patterns of circRNAs during mammalian lung development are largely unexplored.
- Understanding circRNA expression is vital for elucidating developmental mechanisms.
Purpose of the Study:
- To investigate circRNA expression profiles during rat lung development.
- To identify specific circRNAs involved in fetal and early postnatal lung development.
- To explore the potential functions of these circRNAs in lung morphogenesis.
Main Methods:
- High-throughput circRNA sequencing of rat lung tissues at embryonic day (E) 19, E21, and postnatal day (P) 3.
- Validation of differentially expressed circRNAs using reverse transcription-PCR (RT-PCR) across four time points (E16, E19, E21, P3).
- Bioinformatic analysis including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of parent genes.
Main Results:
- Identification of 375 differentially expressed circRNAs between E19 and E21.
- Identification of 358 differentially expressed circRNAs between E21 and P3.
- Three specific circRNAs (rno_circ:chr7:24777879‑24784993, rno_circ:chr14:14620910‑14624933, and rno_circ:chr3:1988750‑ 1998592) showed consistent differential expression across key developmental stages.
- GO and KEGG analyses suggested roles for these circRNAs in lung development processes.
Conclusions:
- This study provides the first comprehensive profile of circRNA expression during rat lung development.
- Newly identified circRNAs are implicated in the intricate processes of lung morphogenesis.
- These findings contribute to understanding normal lung development and may offer insights into lung developmental disorders.
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