Related Experiment Video
Updated: Jan 20, 2026

Optimization for Sequencing and Analysis of Degraded FFPE-RNA Samples
Published on: June 8, 2020
Complete Genome Sequence of a Chlorobenzene Degrader, Pandoraea pnomenusa MCB032
Hong-Jun Chao1,2, Yuan-Yuan Chen1, Jing Wu1
1School of Biology and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan, China.
Abstract:
Chlorobenzenes are ubiquitously distributed, highly persistent, and toxic environmental contaminants. Pandoraea pnomenusa MCB032 was isolated as a new dominant chlorobenzene-utilizing strain from a functionally stable bioreactor during the treatment of chlorobenzenes when strain Burkholderia sp. JS150 disappeared. In study, we report the complete genome sequence of strain MCB032 which consists of a circular chromosome and three plasmids, which are ~ 6 Mb in length with 5450 open reading frames-12 encoding rRNAs and 77 encoding tRNAs. We further identified 17 putative genes encoding the enzymes involved in the methyl-accepting chemotaxis proteins in sensing chemical gradients during chemotaxis. The annotated complete genome sequence of this strain will provide genetic insights into the degradation of chlorinated aromatic compounds. The information will empower the elucidation of chlorobenzene affinity hierarchy and species succession in the bioreactor.
Related Concept Videos
Genomics
Proteins: From Genes to Degradation
Transcription is the synthesis of RNA...
Proteins: From Genes to Degradation
Regulated Protein Degradation
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
Regulated Protein Degradation
Hydrolysis of Chlorobenzene to Phenol: Dow Process

