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Updated: Jan 20, 2026

Confocal Microscopy Reveals Cell Surface Receptor Aggregation Through Image Correlation Spectroscopy
Published on: August 2, 2018
Coincidence Analysis of Molecular Dynamics by Raster Image Correlation Spectroscopy
David F Moreno1, Martí Aldea2,3
1Molecular Biology Institute of Barcelona, IBMB-CSIC, Barcelona, Catalonia, Spain.
Abstract:
The dynamics of cellular processes is a crucial aspect to consider when trying to understand cell function, particularly with regard to the coordination of complex mechanisms involving extensive molecular networks in different cell compartments. Thus, there is an urgent demand of methodologies able to obtain accurate spatiotemporal information on molecular dynamics in live cells. Different variants based on fluorescence correlation spectroscopy have been used successfully in the analysis of protein diffusion and complex or aggregation status. However, the available approaches are limited when simultaneous spatial and temporal resolutions are required to analyze fast processes. Here we describe the use of raster image correlation spectroscopy to analyze the spatiotemporal coincidence of collaborating proteins in highly dynamic molecular mechanisms.
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