Related Experiment Video

Updated: Jan 20, 2026

Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System
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Rapid and Specific Detection of Acinetobacter baumannii Infections Using a Recombinase Polymerase Amplification/Cas12a-based System

Published on: April 25, 2025

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Adjustment of Modified Carbapenem Inactivation Method Conditions for Rapid Detection of Carbapenemase-Producing

Thao Nguyen Vu1,2, Jung Hyun Byun1,3, Roshan D'Souza1,4

  • 1Department of Laboratory Medicine and Research Institute of Bacterial Resistance, Yonsei University College of Medicine, Seoul, Korea.

Annals of Laboratory Medicine
|August 22, 2019
PubMed
Abstract

Insights

Optimized modified carbapenem inactivation methods (mCIMs) accurately detect carbapenemase-producing Acinetobacter baumannii. Tryptic soy broth ensures meropenem disk stability for reliable results.

Area of Science:

  • Microbiology
  • Antimicrobial Resistance
  • Clinical Diagnostics

Background:

  • Modified carbapenem inactivation methods (mCIMs) are crucial for detecting carbapenemase production.
  • Existing mCIMs are not suitable for Acinetobacter baumannii detection.
  • Carbapenemase-producing A. baumannii poses a significant public health threat.

Purpose of the Study:

  • To evaluate the impact of different matrices on disk stability in mCIMs and CIMTris.
  • To optimize mCIM conditions for detecting carbapenemase-producing A. baumannii.
  • To establish a reliable diagnostic method for this challenging pathogen.

Main Methods:

  • Seventy-three A. baumannii isolates were tested using mCIM and CIMTris.
  • The stability of meropenem (MEM) disks in Tryptic soy broth (TSB) and Tris-HCl was assessed.
  • mCIM conditions were modified to improve sensitivity and specificity.

Main Results:

  • Matrices significantly affected MEM disk stability.
  • TSB proved to be a suitable matrix for mCIM, preserving MEM activity.
  • Tris-HCl led to loss of MEM activity in CIMTris.
  • The optimized mCIM achieved 100% sensitivity and specificity.

Conclusions:

  • Optimal mCIM conditions were established for A. baumannii.
  • The developed method is simple, accurate, and reproducible.
  • This advancement aids in the rapid identification of carbapenemase producers.
Keywords:
Acinetobacter baumanniiCIMTrisCarbapenemaseModified carbapenem inactivation method

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