Downregulated long non-coding RNA LINC00899 inhibits invasion and migration of spinal ependymoma cells via
Qun-Bang Chen1, Zhao-Hui Li1, Yao Fu1
1Department of Neurosurgery, China-Japan Union Hospital of Jilin University , Changchun , P.R. China.
Abstract:
This study is aimed to clarify the potential role of lncRNA LINC00899 in invasion and migration of spinal ependymoma cells through the FoxO pathway via RBL2. Spinal ependymoma related chip data (GSE50161 and GSE66354) was initially downloaded and differentially expressed lncRNAs were screened out. Fifty-eight cases of spinal ependymoma and normal ependymal tissues were collected. The effects of LINC00899 and RBL2 on the spinal ependymoma cell migration and invasion were determined using the third generation spinal ependymoma cells and transfection with LINC00899 vector, siRNA-LINC00899 and siRNA-RBL2. The expression of LINC00899, pathway and cell proliferation- and apoptosis-related factors was determined. Finally, we also detected cell proliferation, migration, invasion, cycle and apoptosis after transfection. Our results showed that LINC00899 was up-regulated in spinal ependymoma and RBL2 was confirmed as a target gene of LINC00899 and found to be involved in regulation of FoxO pathway. LINC00899 expression increased in spinal ependymoma tissues whereas RBL2 expression decreased. Moreover, we found that siRNA-LINC00899 could elevate RBL2, p21, p27 and Bax levels, decrease FoxO, Bcl-2, Vimentin, Annexin levels, reduced cell proliferation, migration and invasion and enhanced apoptosis. Taken together, our study suggests that down-regulated LINC00899 exerts anti-oncogenic effects on spinal ependymoma via RBL2-dependent FoxO, which provides a novel therapeutic target for the treatment of spinal ependymomas.
Insights
Down-regulating long non-coding RNA LINC00899 inhibits spinal ependymoma cell invasion and migration. This occurs via the RBL2-dependent FoxO pathway, offering a potential therapeutic target for spinal ependymomas.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Spinal ependymomas are primary tumors of the central nervous system.
- The molecular mechanisms underlying spinal ependymoma progression, including invasion and migration, require further elucidation.
Purpose of the Study:
- To investigate the role of long non-coding RNA LINC00899 in spinal ependymoma.
- To explore the involvement of the FoxO pathway and RBL2 in LINC00899-mediated regulation of spinal ependymoma cell behavior.
Main Methods:
- Analysis of spinal ependymoma chip data (GSE50161, GSE66354) to identify differentially expressed lncRNAs.
- In vitro experiments using spinal ependymoma cell lines with LINC00899 and RBL2 manipulation (vector transfection, siRNA).
- Assessment of cell proliferation, migration, invasion, cell cycle, and apoptosis, along with key protein and pathway marker expression.
Main Results:
- LINC00899 was found to be upregulated in spinal ependymoma tissues and cells.
- RBL2 was identified as a target gene of LINC00899, negatively regulating its expression and participating in the FoxO pathway.
- Downregulation of LINC00899 via siRNA led to increased RBL2, p21, p27, and Bax, decreased FoxO, Bcl-2, Vimentin, and Annexin, resulting in reduced proliferation, migration, invasion, and enhanced apoptosis.
Conclusions:
- LINC00899 promotes spinal ependymoma progression by suppressing RBL2 and inhibiting the FoxO pathway.
- Downregulation of LINC00899 exhibits anti-oncogenic effects in spinal ependymomas.
- LINC00899 represents a potential therapeutic target for spinal ependymoma treatment.
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