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Molecular validation of anthropophilic Phlebotominae sandflies (Diptera: Psychodidae) in Central Panama
Larissa Dutari1,2, Jose R Loaiza1,3,4
1Instituto de Investigaciones Científicas y Servicios de Alta Tecnología, Ciudad del Saber, República de Panamá.
Abstract:
Six Phlebotominae sand fly species are incriminated as biological vectors of human pathogens in Panama, but molecular corroboration is still needed. We aim at confirming the identity of Phlebotominae species documented as anthropophilic in Panama. Adult sandflies were collected from August 2010 to February 2012 in Central Panama using CDC light traps. Species confirmation was accomplished through molecular barcodes and allied sequences from GenBank. A total of 53,366 sand fly specimens representing 18 species were collected. Five species were validated molecularly as single phylogenetic clusters, but Psychodopygus thula depicted two genetically divergent lineages, which may be indicative of cryptic speciation.
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