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Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models
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miR-1301/TRIAP1 Axis Participates in Epirubicin-Mediated Anti-Proliferation and Pro-Apoptosis in Osteosarcoma

Lijun Yu1, Min Meng1, Yun Bao1

  • 1Department of Pharmacy, Gansu Provincial Hospital, Lanzhou, Gansu, China.

Yonsei Medical Journal
|August 22, 2019
PubMed
Abstract

Insights

MicroRNA-1301 (miR-1301) enhances osteosarcoma cell sensitivity to epirubicin by downregulating TRIAP1. This finding provides a novel therapeutic target for improving epirubicin efficacy in osteosarcoma treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Osteosarcoma is a primary bone malignancy with limited treatment options.
  • Epirubicin is a key chemotherapeutic agent, but resistance remains a challenge.
  • MicroRNA-1301 (miR-1301) is implicated in osteosarcoma development, but its role in drug sensitivity is unclear.

Purpose of the Study:

  • To investigate the role of miR-1301 in osteosarcoma chemosensitivity to epirubicin.
  • To elucidate the molecular mechanism underlying miR-1301's effect on epirubicin treatment.

Main Methods:

  • Osteosarcoma cell lines (U2OS, SAOS-2) were treated with epirubicin.
  • Cell proliferation and apoptosis were assessed using CCK-8 assay and flow cytometry.
  • Expression levels of apoptosis markers (Bcl-2, Bax, cleaved-caspase-3, cleaved-PARP1) and miR-1301 were analyzed by qRT-PCR and Western blot.
  • The regulatory relationship between miR-1301 and TRIAP1 was confirmed via luciferase reporter assay.

Main Results:

  • Epirubicin inhibited cell proliferation and induced apoptosis in a dose-dependent manner.
  • miR-1301 expression was downregulated in osteosarcoma cells and significantly upregulated by epirubicin.
  • Overexpression of miR-1301 enhanced epirubicin's anti-cancer effects, while depletion attenuated them.
  • miR-1301 directly targeted and downregulated TRIAP1 expression, mediating epirubicin's inhibitory effects on proliferation.

Conclusions:

  • miR-1301 plays a crucial role in mediating osteosarcoma cell sensitivity to epirubicin.
  • The miR-1301/TRIAP1 axis is a key pathway involved in epirubicin's efficacy.
  • Targeting miR-1301 or TRIAP1 may represent a promising strategy to overcome epirubicin resistance in osteosarcoma.
Keywords:
TRIAP1epirubicinmiR-1301osteosarcoma

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