Related Experiment Video
Updated: Jan 20, 2026

Isolation and Expansion of Neurospheres from Postnatal (P1−3) Mouse Neurogenic Niches
Published on: May 23, 2020
EGFR Signaling Termination via Numb Trafficking in Ependymal Progenitors Controls Postnatal Neurogenic Niche
Khadar Abdi1, Gabriel Neves1, Joon Pyun1
1Department of Cell Biology, Duke University, School of Medicine, Durham, NC 27710, USA.
Abstract:
Specialized microenvironments, called niches, control adult stem cell proliferation and differentiation. The brain lateral ventricular (LV) neurogenic niche is generated from distinct postnatal radial glial progenitors (pRGPs), giving rise to adult neural stem cells (NSCs) and niche ependymal cells (ECs). Cellular-intrinsic programs govern stem versus supporting cell maturation during adult niche assembly, but how they are differentially initiated within a similar microenvironment remains unknown. Using chemical approaches, we discovered that EGFR signaling powerfully inhibits EC differentiation by suppressing multiciliogenesis. We found that EC pRGPs actively terminated EGF activation through receptor redistribution away from CSF-contacting apical domains and that randomized EGFR membrane targeting blocked EC differentiation. Mechanistically, we uncovered spatiotemporal interactions between EGFR and endocytic adaptor protein Numb. Ca2+-dependent basolateral targeting of Numb is necessary and sufficient for proper EGFR redistribution. These results reveal a previously unknown cellular mechanism for neighboring progenitors to differentially engage environmental signals, initiating adult stem cell niche assembly.
Related Concept Videos
Physiology of Respiration II: Neurogenic Control of Respiration
Central Control
The brainstem is the primary site of central control, hosting respiratory centers:
Ecological Niches
Differentiation of Common Myeloid Progenitor Cells
Termination of Translation
Termination of Translation
Stem Cell Niche

