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Related Concept Videos

Embryonic Stem Cells00:58

Embryonic Stem Cells

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Embryonic Stem Cells00:57

Embryonic Stem Cells

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Embryonic stem (ES) cells were first discovered in mice in 1981 by Martin Evans. In 1998, James Thomson identified a method to isolate embryonic stem cells from humans. Human embryonic stem cells (hESCs) are obtained from 3-5 day old embryos that remain unused after an in vitro fertilization procedure.
ES cells are grown in a culture medium where they can divide indefinitely, creating ES cell lines. Under certain conditions, ES cells can differentiate, either spontaneously into a variety of...
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Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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Adult Stem Cells01:33

Adult Stem Cells

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Transcription Factors02:16

Transcription Factors

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Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
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Molecular Factors Affecting Cell Division01:27

Molecular Factors Affecting Cell Division

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Several external and internal factors influence the initiation and inhibition of cell division. For instance, the death of nearby cells or the release of human growth hormone (hGH) promotes cell division. In contrast, lack of hGH or crowding of cells can inhibit cell division.
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Related Experiment Video

Updated: Jan 20, 2026

Electrically Conductive Scaffold to Modulate and Deliver Stem Cells
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Electrically Conductive Scaffold to Modulate and Deliver Stem Cells

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SCF (Stem Cell Factor) and cKIT Modulate Pathological Ocular Neovascularization.

Koung Li Kim1, Songyi Seo1, Jee Taek Kim2

  • 1From the College of Pharmacy (K.L.K., S.S., Y.Y., W.S.), Chung-Ang University, Seoul, Korea.

Arteriosclerosis, Thrombosis, and Vascular Biology
|August 23, 2019
PubMed
Summary
This summary is machine-generated.

Aberrant neovascularization, a cause of blindness, involves cKIT and stem cell factor (SCF). Blocking these targets significantly suppressed pathological ocular neovascularization in mouse models.

Keywords:
angiogenesiscKITcateninendothelial cellhypoxiastem cell factor

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In Vivo Multimodal Imaging and Analysis of Mouse Laser-Induced Choroidal Neovascularization Model
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In Vivo Multimodal Imaging and Analysis of Mouse Laser-Induced Choroidal Neovascularization Model

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Area of Science:

  • Ophthalmology
  • Molecular Biology
  • Cell Biology

Background:

  • Aberrant neovascularization causes blindness in diseases like age-related macular degeneration and diabetic retinopathy.
  • Identifying key regulators of pathological ocular neovascularization is crucial for therapeutic development.

Purpose of the Study:

  • To investigate the role of cKIT and its ligand, stem cell factor (SCF), in pathological ocular neovascularization.
  • To explore SCF/cKIT signaling pathways in angiogenesis.

Main Methods:

  • Utilized oxygen-induced retinopathy and laser-induced choroidal neovascularization murine models.
  • Examined cKIT and SCF expression under hypoxia and normoxia.
  • Employed cKit mutant mice and anti-SCF neutralizing IgG for blockade studies.
  • Investigated downstream signaling via glycogen synthase kinase-3β and β-catenin.

Main Results:

  • Hypoxia upregulated cKIT in endothelial cells, enhancing angiogenic response to SCF.
  • cKIT and SCF expression increased in ocular tissues during pathological neovascularization.
  • Blocking cKIT/SCF substantially suppressed neovascularization in murine models.
  • SCF/cKIT signaling promoted neovascularization via β-catenin nuclear translocation and target gene transcription.

Conclusions:

  • SCF and cKIT play a significant role in pathological ocular neovascularization.
  • SCF/cKIT signaling, mediated by β-catenin, drives angiogenesis.
  • SCF and cKIT represent promising novel therapeutic targets for vision-threatening ocular neovascular diseases.