Related Experiment Video

Updated: Jan 20, 2026

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR
09:03

Profiling Individual Human Embryonic Stem Cells by Quantitative RT-PCR

Published on: May 29, 2014

12.0K

An altered left ventricle protein profile in human ischemic cardiomyopathy revealed in comparative quantitative

Xin Yi1,2,3, Ding-Sheng Jiang4,5,6, Gaoke Feng1,2,3

  • 1Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, People’s Republic of China

Kardiologia Polska
|August 23, 2019
PubMed

Insights

Researchers identified key proteins altered in ischemic cardiomyopathy (ICM). These differentially expressed proteins, including C1 inhibitor SERPING1, offer potential new diagnostic and therapeutic targets for heart failure patients.

Area of Science:

  • Cardiology
  • Proteomics
  • Biochemistry

Background:

  • Ischemic cardiomyopathy (ICM) is a primary cause of heart failure, stemming from coronary artery disease.
  • Identifying and quantifying protein expression changes in ICM patients is crucial for developing better diagnostic and treatment strategies.

Purpose of the Study:

  • To identify and quantify differentially expressed proteins in the left ventricles of patients with ICM.
  • To explore the molecular mechanisms underlying ICM through proteomic analysis.

Main Methods:

  • Quantitative proteomic analysis of left ventricular tissue from 6 ICM patients and 7 normal heart donors using liquid chromatography-tandem mass spectrometry.
  • Bioinformatic analyses including gene ontology, pathway mapping, and protein interaction analysis were performed on identified proteins.

Main Results:

  • 1723 proteins were quantified, with 104 upregulated and 63 downregulated in ICM patients.
  • Altered proteins were associated with extracellular matrix, metabolism, immune response, muscle contraction, cytoskeleton, transcription/translation, and signal transduction.
  • C1 inhibitor SERPING1 was found to inhibit complement activation in response to ischemic stimulus.

Conclusions:

  • The study identified differentially expressed proteins that are potential novel diagnostic and therapeutic targets for ICM.
  • These findings advance the understanding of molecular mechanisms in ICM and highlight SERPING1's role in complement regulation.
Abstract

More Related Videos

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue11:49

Quantitative Proteomics Workflow using Multiple Reaction Monitoring Based Detection of Proteins from Human Brain Tissue

Published on: August 28, 2021

5.0K
Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques08:07

Author Spotlight: Advancing Antiviral Strategies Through Novel Immunocapture and Mass Spectrometry Techniques

Published on: January 12, 2024

1.2K

Related Concept Videos

Cardiomyopathy II: Dilated Cardiomyopathy01:30

Cardiomyopathy II: Dilated Cardiomyopathy

Dilated cardiomyopathy, or DCM, is a progressive myocardial disorder characterized by ventricular chamber dilation and contractile dysfunction.EtiologyVarious factors can cause DCM, including hypertension and heavy alcohol intake, which contribute to the weakening and enlargement of the heart muscle. Viral infections, such as Coxsackievirus B, adenoviruses, and influenza, can lead to DCM by causing inflammation and damage to heart tissue. Certain chemotherapeutic agents, including daunorubicin,...
489
Cardiomyopathy IV: Restrictive Cardiomyopathy01:29

Cardiomyopathy IV: Restrictive Cardiomyopathy

Restrictive cardiomyopathy (RCM) is a rare heart muscle disease characterized by impaired ventricular filling due to stiffened ventricular walls, leading to significant diastolic dysfunction.EtiologyRestrictive cardiomyopathy can arise from both inherited and acquired diseases, many of which are systemic. It is categorized into four main types: infiltrative, storage, non-infiltrative, and endomyocardial diseases.Infiltrative diseases, such as amyloidosis, lead to RCM by depositing amyloid...
481
Cardiomyopathy III: Hypertrophic Cardiomyopathy01:29

Cardiomyopathy III: Hypertrophic Cardiomyopathy

Hypertrophic cardiomyopathy, or HCM, is an autosomal dominant genetic disorder characterized by asymmetric left ventricular hypertrophy without ventricular dilation. It is more common in men and is typically diagnosed in young, athletic adults.EtiologyHCM is primarily genetic and is caused by mutations in genes encoding sarcomeric proteins. Researchers have identified over 1400 mutations across at least 11 different genes. Among these, the most frequently occurring mutations are found in the...
418
Cardiomyopathy I: Introduction and Classification01:25

Cardiomyopathy I: Introduction and Classification

Cardiomyopathy, or CMP, is a group of diseases affecting the myocardial structure, impairing its ability to pump blood effectively. This condition can lead to arrhythmias, heart failure, or sudden cardiac death.Cardiomyopathies are classified into primary and secondary categories:Primary Cardiomyopathy refers to conditions involving only the heart muscle that are often idiopathic (of unknown cause) or genetic. They primarily affect the myocardium without the involvement of other systemic...
517
Cardiomyopathy VI: Nursing Management01:29

Cardiomyopathy VI: Nursing Management

Assessment: Nursing management of patients with cardiomyopathy begins with a thorough assessment of the patient's history, including a family history of cardiomyopathy or sudden cardiac death, personal history of heart disease, hypertension, diabetes, and any alcohol consumption or drug use.During the physical examination, assess vital signs, look for signs of heart failure (such as edema, jugular venous distention, and cyanosis), auscultate for abnormal heart sounds (like murmurs and gallops),...
318
Anatomy of the Brain: Ventricles01:18

Anatomy of the Brain: Ventricles

There are hollow fluid-filled cavities known as ventricles deep inside the human brain. There are two lateral ventricles, one in each cerebral hemisphere, and each has three different projections — the anterior, inferior, and posterior horns visible from the lateral side. A thin membrane called the septum pellucidum separates the two lateral ventricles. The slender third ventricle in the diencephalon is connected to each lateral ventricle via a channel called the interventricular foramen.
8.3K