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Comparative immunochemical studies of carbonic anhydrase III in horses and other mammalian species
1Laboratory of Veterinary Physiology, School of Veterinary Medicine, Azabu University, Kanagawa, Japan.
Abstract:
1. Carbonic anhydrase III (CA-III) from different mammalian species (horse, cow, dog, cat, rat and rabbit) has been analyzed by the immunodiffusion technique with anti-equine CA-III serum. 2. Immunodiffusion demonstrated the absence of cross-reactivity between isozyme CA-I, CA-II, and CA-III. 3. Cross-reactions were observed between the CA-III from all the species examined except the rabbit. 4. Molecular weights and isoelectric points of CA-III from different species were determined by Western blotting.