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Protective effect of vitamin E on oxidative stress and sperm apoptosis in diabetic Mice

Khadijeh Mirzaei Khorramabadi1, Ali Reza Talebi2, Abolghasem Abbasi Sarcheshmeh3

  • 1International Campus, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

International Journal of Reproductive Biomedicine
|August 23, 2019
PubMed
Abstract

Insights

Vitamin E supplementation protects male reproductive health from diabetes-induced oxidative stress. This study shows vitamin E improves sperm quality and testicular function in diabetic mice.

Area of Science:

  • Reproductive Biology
  • Endocrinology
  • Oxidative Stress Research

Background:

  • Diabetes mellitus is a significant contributor to male reproductive dysfunction.
  • Oxidative stress and free radical generation are key mechanisms underlying these disorders.

Purpose of the Study:

  • To investigate the protective effects of vitamin E against diabetes-induced oxidative stress in the male reproductive system.
  • To evaluate vitamin E's impact on testicular function and sperm parameters in a mouse model.

Main Methods:

  • Diabetes was induced in adult male mice using streptozotocin.
  • Mice received daily intraperitoneal injections of vitamin E for 30 days.
  • Testicular tissues were analyzed for sperm count, motility, morphology, oxidative stress markers (MDA, TAC), and apoptosis (TUNEL assay).

Main Results:

  • Diabetic mice exhibited significantly reduced sperm count, motility, and normal morphology, alongside increased oxidative damage (lower TAC, higher MDA) and apoptosis compared to controls.
  • Vitamin E treatment significantly improved sperm parameters, increased antioxidant capacity (TAC), and reduced oxidative stress markers and apoptosis in diabetic mice.
  • These improvements demonstrate vitamin E's efficacy in mitigating diabetes-related testicular damage.

Conclusions:

  • Vitamin E demonstrates significant protective effects on the male reproductive system against diabetes-induced oxidative stress.
  • Vitamin E may serve as a therapeutic agent to preserve sperm quality and testicular function in individuals with diabetes mellitus.
Keywords:
Diabetes treatmentMale reproductive dysfunctionCase-control studyDiabetic Syrian miceVitamin E

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