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Effects of different doses of tamoxifen on the sperm parameters and chromatin quality in mice: An experimental model

Sepideh Sadeghi1, Ali Reza Talebi2, Abbas Shahedi1

  • 1Department of Biology and Anatomical Sciences, Shahid Sadoughi University of Medical Sciences, Yazd, Iran.

International Journal of Reproductive Biomedicine
|August 23, 2019
PubMed
Abstract

Insights

Tamoxifen (TX) treatment for male infertility may increase abnormal sperm chromatin. This study found TX elevated abnormal sperm morphology and chromatin in mice, especially at higher doses.

Area of Science:

  • Reproductive biology
  • Endocrinology
  • Toxicology

Background:

  • Tamoxifen (TX) is a common treatment for male infertility.
  • Previous research suggests TX can improve male fertility by inducing sperm production.

Purpose of the Study:

  • To investigate the impact of varying Tamoxifen doses on mouse sperm parameters.
  • To assess the effects of Tamoxifen on sperm chromatin quality in mice.

Main Methods:

  • NMRI mice were administered Tamoxifen (0.4 mg/kg/day or 0.6 mg/kg/day) or vehicle for 35 days.
  • Epididymal spermatozoa were analyzed for count, motility, viability, morphology, and chromatin integrity.
  • Aniline Blue (AB) and Toluidine Blue (TB) staining were used to evaluate chromatin quality.

Main Results:

  • Tamoxifen did not significantly alter sperm count, motility, or viability.
  • Both low and high doses of TX increased the percentage of sperm with abnormal morphology and chromatin.
  • A significant increase in spermatozoa with residual histones was observed at higher TX doses.

Conclusions:

  • Tamoxifen use in male infertility treatment may elevate the risk of spermatozoa with abnormal chromatin.
  • The observed effects appear to be dose-dependent.
  • Further research is needed to confirm these findings in humans.
Keywords:
ChromatinMiceSpermTamoxifen

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