Related Experiment Video

Updated: Jan 20, 2026

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells
09:32

Multiplexed Immunofluorescence Analysis and Quantification of Intratumoral PD-1+ Tim-3+ CD8+ T Cells

Published on: February 8, 2018

15.4K

Tim-3 expression in glioma cells is associated with drug resistance

Ji Zhang1, Zheng Quan Zhu2, Yan Xia Li3

  • 1Department of Neurosurgery, State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine, Sun Yat-sen University Cancer Center, Guangzhou, China.

Journal of Cancer Research and Therapeutics
|August 23, 2019
PubMed
Abstract

Insights

T-cell immunoglobulin and mucin-domain containing-3 (Tim-3) is present in glioma cells and hinders temozolomide (TMZ) treatment. Inhibiting Tim-3 enhances TMZ

Area of Science:

  • Neuro-oncology
  • Immunology
  • Molecular Biology

Background:

  • T-cell immunoglobulin and mucin-domain containing-3 (Tim-3) negatively regulates antitumor immunity.
  • The role of Tim-3 in glioma treatment resistance is not fully understood.

Purpose of the Study:

  • To investigate Tim-3 expression in glioma cells.
  • To evaluate the impact of Tim-3 on drug resistance in glioma.
  • To explore Tim-3's role in temozolomide (TMZ) efficacy.

Main Methods:

  • U87 and U251 glioma cell lines were used.
  • Cell proliferation, viability, and Tim-3 expression (mRNA and protein) were assessed.
  • Tim-3 expression was inhibited using small interfering RNA (siRNA) to test chemosensitivity.

Main Results:

  • Tim-3 mRNA and protein were detected in glioma cells.
  • Higher Tim-3 expression was observed in living cells with increasing temozolomide (TMZ) concentration.
  • Inhibiting Tim-3 with siRNA significantly enhanced TMZ's cytotoxic effect by increasing apoptosis.

Conclusions:

  • Tim-3 is expressed in glioma cells and contributes to drug resistance.
  • Knocking down Tim-3 expression improves the efficacy of temozolomide (TMZ) treatment in gliomas.
Keywords:
Drug resistanceTim-3glioma cellsmall interfering RNAs

More Related Videos

Author Spotlight: An Efficient Methodology to Confidently Differentiate and Characterize Fentanyl Analogs10:13

Author Spotlight: An Efficient Methodology to Confidently Differentiate and Characterize Fentanyl Analogs

Published on: November 8, 2024

2.8K
Author Spotlight: Applications of TEER Detection to Assess Cell Barrier Integrity09:03

Author Spotlight: Applications of TEER Detection to Assess Cell Barrier Integrity

Published on: September 29, 2023

4.7K

Related Concept Videos

Cell Specific Gene Expression01:58

Cell Specific Gene Expression

Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.3K
Cell Specific Gene Expression01:58

Cell Specific Gene Expression

5.4K
Resistivity01:22

Resistivity

When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.4K
Resistance01:19

Resistance

When a current moves through any conductor, the conductor causes some level of difficulty for the current to flow. The measure of that difficulty is known as the resistance of the material and is represented by R. Every material has its own resistance. In the case of conductors, heat is emitted whenever a current passes through them. Resistance depends on the resistivity of the material. Resistivity is a characteristic of the material used to fabricate electrical components, whereas the...
5.7K
Resistance and Conductance01:25

Resistance and Conductance

A conductor's DC resistance at a given temperature is influenced by its resistivity, length, and cross-sectional area. Resistivity is an inherent property of the conductor material, with annealed copper serving as the international standard for measurement. For instance, the resistivity of hard-drawn aluminum at 20 degrees Celsius is 61% of the standard conductivity of annealed copper.
Various factors impact the resistance of a conductor. Spiraling in stranded conductors increases their...
492
Equivalent Resistance01:16

Equivalent Resistance

In circuit analysis, situations often arise where resistors are neither in series nor parallel configurations. To tackle such scenarios, three-terminal equivalent networks like the wye (Y) (Figure 1 (a)) or tee (T) and delta (Δ) (Figure 1 (b)) or pi (π) networks come into play. These networks offer versatile solutions and are frequently encountered in various applications, including three-phase electrical systems, electrical filters, and matching networks.
939