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Related Experiment Videos

Markers shared between dorsal root and enteric ganglia.

A L Kirchgessner1, J Dodd, M D Gershon

  • 1Department of Anatomy, College of Physicians and Surgeons, Columbia University, New York, New York 10032.

The Journal of Comparative Neurology
|October 22, 1988
PubMed
Summary

Researchers identified potential markers for intrinsic sensory neurons in the rat gut using specific antibodies and enzyme activity tests. These findings suggest that sensory neurons similar to those in dorsal root ganglia may exist within the intestine.

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Area of Science:

  • Neuroscience
  • Gastroenterology
  • Cell Biology

Background:

  • The bowel contains intrinsic primary afferent neurons with mucosal projections, but these cells have not been definitively identified.
  • Sensory neurons in rat dorsal root ganglia (DRG) are characterized by specific carbohydrate differentiation antigens and enzymatic markers.

Purpose of the Study:

  • To determine if carbohydrate differentiation antigens or enzymatic markers common to primary sensory neurons are present in enteric neurons.
  • To investigate the coincident expression of these markers with neuropeptides in rat small intestine neurons.

Main Methods:

  • Utilized monoclonal antibodies (MAbs) 1B2/1B7 and alpha-SSEA-1 to identify neuronal subsets.
  • Assessed carbonic anhydrase (CA) and fluoride-resistant acid phosphatase (FRAP) activities in enteric neurons.

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  • Examined co-localization of identified neuronal subsets with neuropeptides like VIP, NPY, SP, and CGRP.
  • Administered capsaicin neonatally to assess the impact on neuronal markers.

    • Main Results:

    • MAb 1B2/1B7 identified subsets in submucosal (approx. 46%) and myenteric (approx. 2%) plexuses; these neurons projected to the mucosa and expressed VIP and NPY.
    • Alpha-SSEA-1 identified a small subset in the myenteric plexus, unlikely to be primary afferents, expressing SOM or ENK.
    • CA activity was prominent in submucosal neurons (approx. 39%) and mucosal nerve fibers; some CA+ neurons were CGRP+.
    • Neonatal capsaicin treatment eliminated 1B2/1B7 immunoreactivity in submucosal neurons, but not VIP.

    Conclusions:

    • The MAb 1B2/1B7 and CA activity mark distinct populations of submucosal neurons.
    • While not definitive, the presence of these markers in the gut is consistent with the existence of intrinsic sensory neurons related to DRG neurons.
    • Further research is needed to define the functional and developmental significance of these enteric sensory neurons.