Recent advances in plasmid-based tools for establishing novel microbial chassis.

Area of Science:

• Synthetic biology
• Microbial biotechnology

Background:

• Genetic tools have advanced synthetic biology, enabling complex genome engineering.
• Traditional microbial models like E. coli and S. cerevisiae are limited for specific biotechnological applications.
• Shifting to non-conventional microbial chassis with inherent capacities is a key challenge.

Purpose of the Study:

• To review recent advances in plasmid-based molecular tools for engineering microbial cell factories.
• To provide a guide for developing custom microbial hosts.
• To facilitate the domestication of alternative microorganisms for biotechnology.

Main Methods:

• Review of recent literature on plasmid-based tools and genome engineering techniques.
• Focus on homologous recombination, CRISPR/Cas9, and other novel methods.
• Discussion of tools for both narrow and broad host ranges.

Main Results:

• Significant progress has been made in developing plasmid-based tools for synthetic biology.
• Novel genome engineering methods are expanding the possibilities for microbial host development.
• The development of "build-your-own" microbial host guides aids researchers.

Conclusions:

• Innovative technologies are essential for harnessing the biotechnological potential of alternative microorganisms.
• Advanced molecular tools and genome engineering strategies are enabling the creation of specialized microbial cell factories.
• This work supports the transition to non-conventional microbial chassis for diverse biotechnological tasks.