Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Matrix-Assisted Laser Desorption Ionization (MALDI)01:08

Matrix-Assisted Laser Desorption Ionization (MALDI)

1.0K
Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI is an ionization technique, widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix...
1.0K
Bioequivalence Experimental Study Designs: Repeated Measures, Cross-Over, Carry-Over, and Latin Square Designs01:15

Bioequivalence Experimental Study Designs: Repeated Measures, Cross-Over, Carry-Over, and Latin Square Designs

188
Body:Bioequivalence experimental study designs play a pivotal role in testing the effectiveness of various treatments. Key among these are the repeated measures, cross-over, carry-over, and Latin square designs. In the repeated measures design, each subject receives all treatments, allowing for temporal comparisons. This type of design is useful in reducing variability but requires careful planning to avoid bias.The cross-over design, an economical method, involves sequential administration of...
188
Detection of Gross Error: The Q Test01:00

Detection of Gross Error: The Q Test

6.9K
When one or more data points appear far from the rest of the data, there is a need to determine whether they are outliers and whether they should be eliminated from the data set to ensure an accurate representation of the measured value. In many cases, outliers arise from gross errors (or human errors) and do not accurately reflect the underlying phenomenon. In some cases, however, these apparent outliers reflect true phenomenological differences. In these cases, we can use statistical methods...
6.9K
Detection of Black Holes01:10

Detection of Black Holes

2.5K
Although black holes were theoretically postulated in the 1920s, they remained outside the domain of observational astronomy until the 1970s.
Their closest cousins are neutron stars, which are composed almost entirely of neutrons packed against each other, making them extremely dense. A neutron star has the same mass as the Sun but its diameter is only a few kilometers. Therefore, the escape velocity from their surface is close to the speed of light.
Not until the 1960s, when the first neutron...
2.5K
Difference from Background: Limit of Detection01:05

Difference from Background: Limit of Detection

8.0K
The limit of detection (LOD) is the smallest amount of analyte that can be distinguished from the background noise. The LOD value corresponds to the concentration at which the analyte signal is three times larger than the standard deviation of the blank signal. Below this value, the analyte signal cannot be differentiated from the background noise. It is calculated by dividing the calibration slope by 3 times the standard deviation of the blank signals.
The LOD indicates the presence or absence...
8.0K
Effects of EDTA on End-Point Detection Methods01:18

Effects of EDTA on End-Point Detection Methods

627
Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...
627

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Luminescence-guided and visibly transparent solar concentrators based on silicon quantum dots.

Optics express·2022
Same author

A bioactive poly(ether-ether-ketone) nanocomposite scaffold regulates osteoblast/osteoclast activity for the regeneration of osteoporotic bone.

Journal of materials chemistry. B·2022
Same author

Efficacy and safety of nivolumab for advanced/recurrent non-small-cell lung cancer: an up-to-date meta-analysis of large-scale phase III randomized controlled trials.

Future oncology (London, England)·2022
Same author

Sudden severe hypotension following autologous blood transfusion: A case report.

Asian journal of surgery·2022
Same author

Pyrocatechol Alleviates Cisplatin-Induced Acute Kidney Injury by Inhibiting ROS Production.

Oxidative medicine and cellular longevity·2022
Same author

Identification and validation of a novel necroptosis-related prognostic signature in cervical squamous cell carcinoma and endocervical adenocarcinoma.

Frontiers in oncology·2022

Related Experiment Video

Updated: Jan 20, 2026

Flow-assisted Dielectrophoresis: A Low Cost Method for the Fabrication of High Performance Solution-processable Nanowire Devices
09:14

Flow-assisted Dielectrophoresis: A Low Cost Method for the Fabrication of High Performance Solution-processable Nanowire Devices

Published on: December 7, 2017

8.2K

Nanowire assisted repeatable DEP-SERS detection in microfluidics.

Tingting Ge1, Sheng Yan2, Lingjun Zhang1

  • 1Chongqing Key Laboratory of Soft Condensed Matter Physics and Smart Materials, College of Physics, Chongqing University, Chongqing, 401331, People's Republic of China.

Nanotechnology
|August 23, 2019
PubMed
Summary
This summary is machine-generated.

This study presents a novel dielectrophoresis-SERS system for enhanced molecular detection in microfluidics. The method utilizes silver nanowire aggregation for high sensitivity and reproducibility, applicable to food safety and environmental monitoring.

More Related Videos

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
06:55

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

Published on: August 4, 2022

2.2K
Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs
03:33

Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs

Published on: November 17, 2023

3.3K

Related Experiment Videos

Last Updated: Jan 20, 2026

Flow-assisted Dielectrophoresis: A Low Cost Method for the Fabrication of High Performance Solution-processable Nanowire Devices
09:14

Flow-assisted Dielectrophoresis: A Low Cost Method for the Fabrication of High Performance Solution-processable Nanowire Devices

Published on: December 7, 2017

8.2K
Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria
06:55

Microfluidics-Assisted Selective Depolarization of Axonal Mitochondria

Published on: August 4, 2022

2.2K
Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs
03:33

Author Spotlight: Development and Application of SERS Flexible Substrates Using Synthesized AgNPs

Published on: November 17, 2023

3.3K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Microfluidics

Background:

  • Surface-enhanced Raman spectroscopy (SERS) offers high sensitivity, miniaturization, and online detection capabilities.
  • Integrating SERS into microfluidic devices presents opportunities for advanced analytical applications.
  • Controlling nanoparticle aggregation is crucial for optimizing SERS signal enhancement.

Purpose of the Study:

  • To develop and demonstrate a novel SERS detection system within microfluidics.
  • To utilize dielectrophoresis (DEP) for controlled aggregation of silver nanowires (AgNWs).
  • To achieve enhanced Raman signal intensities through nanogap formation modulated by DEP.

Main Methods:

  • Fabrication of a microfluidic chip for SERS detection.
  • Application of dielectrophoresis (DEP) to induce aggregation of silver nanowires (AgNWs).
  • Investigation of DEP parameters (voltage, time) for optimal AgNW aggregation.
  • SERS measurements of methylene blue and rhodamine 6G at various concentrations.
  • Assessment of reproducibility, uniformity, and reusability of the DEP-SERS system.
  • Detection of melamine for food safety applications.

Main Results:

  • DEP effectively modulated AgNW aggregation, creating nanogaps that significantly enhanced Raman intensities.
  • Optimal DEP conditions for AgNW aggregation were determined.
  • High reproducibility and uniformity were achieved in SERS detection of target molecules.
  • The DEP-SERS system demonstrated reusability for detecting different molecules.
  • Successful detection of melamine was achieved, indicating potential for food safety analysis.

Conclusions:

  • The developed dielectrophoresis-assisted SERS (DEP-SERS) system in microfluidics provides highly sensitive and reproducible molecular detection.
  • The system's ability to be reused for various analytes highlights its versatility.
  • This technology holds significant promise for applications in environmental monitoring and food safety evaluation.