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Microscopy-based Assays for High-throughput Screening of Host Factors Involved in Brucella Infection of Hela Cells
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Host Prdx6 contributing to the intracellular survival of Brucella suis S2 strain

Lu-Lu Wang1, Xiao-Feng Chen1, Pan Hu1

  • 1Key Laboratory of Zoonosis Research, Ministry of Education, Institute of Zoonosis / College of Veterinary Medicine, Jilin University, Xi An Da Lu 5333, Changchun, 130062, China.

BMC Veterinary Research
|August 24, 2019
PubMed
Abstract

Insights

Host Prdx6 protein enhances Brucella suis S2 survival within macrophages. This study reveals Prdx6

Area of Science:

  • Microbiology
  • Immunology
  • Cell Biology

Background:

  • Brucellosis is a global zoonotic disease caused by Brucella bacteria, which infect macrophages.
  • Mechanisms of Brucella survival within host macrophages remain incompletely understood.
  • Peroxiredoxin 6 (Prdx6) is a bifunctional enzyme involved in oxidative stress defense and apoptosis regulation.

Purpose of the Study:

  • To investigate the role of host Peroxiredoxin 6 (Prdx6) in Brucella infection.
  • To elucidate the impact of Prdx6 on Brucella survival within macrophages.

Main Methods:

  • Expression and purification of recombinant mouse Peroxiredoxin 6 (MmPrdx6).
  • Preparation of monoclonal antibodies against MmPrdx6.
  • Infection of RAW264.7 murine macrophages with Brucella suis S2 strain.
  • Analysis of intracellular Prdx6 expression levels post-infection.
  • Manipulation of Prdx6 expression (overexpression and knockdown) in macrophages.

Main Results:

  • Intracellular Prdx6 expression levels in macrophages initially decreased then increased after Brucella suis S2 infection.
  • Overexpression of Prdx6 in macrophages led to higher intracellular B. suis S2 levels.
  • Knockdown of Prdx6 in macrophages resulted in reduced intracellular B. suis S2 levels.

Conclusions:

  • Host Peroxiredoxin 6 (Prdx6) promotes the intracellular survival of Brucella suis S2.
  • Prdx6 plays a significant role in the host-pathogen interaction during Brucella infection.
Keywords:
BrucellaHostIntracellular survivalMacrophagePrdx6

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