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Updated: Jan 20, 2026
In Ovo Xenografting of Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells (PDX-ALL)
Published on: August 1, 2025
iTRAQ-based quantitative protein expression profiling of biomarkers in childhood B-cell and T-cell acute
Runhong Yu1, Jingyu Zhang2, Yuzhu Zang1
1Department of Hematology, People's Hospital of Zhengzhou University/Henan Provincial People's Hospital, Zhengzhou 450003, Henan, People's Republic of China.
Purpose:
This study screened serum proteins to identify potential biomarkers for childhood B-cell and T-cell acute lymphoblastic leukemia (ALL).
Patients And Methods:
Serum collected from 20 newly diagnosed B-cell ALL, 20 T-cell ALL and 20 healthy children. The peptides from these samples were subjected to iTRAQ. Differentially expressed proteins (DEPs) were further validated by ELISA in 24 B-ALL, 24 T-ALL, and 24 healthy children.
Results:
Bioinformatics analysis revealed several pathways, including atherosclerosis signaling, interleukin signaling and production in macrophages and clathrin-mediated endocytosis signaling, that were closely related to childhood T-cell ALL. Furthermore, four selected proteins, namely LRG1, S100A8, SPARC and sL-selectin, were verified by ELISA. These results were consistent with the results of the proteomics analysis.
Conclusion:
Serum S100A8 may serve as new diagnostic biomarkers in childhood B-cell ALL and T-cell ALL.
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