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Updated: Jan 20, 2026

Visualizing Dengue Virus through Alexa Fluor Labeling
Published on: July 9, 2011
Assays with recombinant soluble isoforms of DC-SIGN, a dengue virus ligand, show variation in their ability to bind
Lailah Horácio Sales Pereira1,2, Thaís Paiva Porto de Souza1, Vidyleison Neves Camargos3
1Laboratory of Molecular Biology, Universidade Federal de São João Del Rei (UFSJ), Divinópolis, Minas Gerais, Brazil.
Abstract:
The DC-SIGN glycoprotein is responsible for the initial adhesion of dengue virus (DENV) to immune cells by the carbohydrate recognition domain (CRD). There are thirteen soluble and membrane-bound DC-SIGN isoforms, but the role of soluble isoforms in the DENV internalization process is not known. Five isoforms with an altered or absent CRD were identified, and three different soluble isoforms were used to confirm the interactions with mannose residues. The results show the loss of binding ability of one soluble isoform and binding ability of two of them. All of them will be used to verify their role in the DENV internalization process.
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