Related Experiment Video
Updated: Jan 20, 2026
05:20
Zebrafish Model of Neuroblastoma Metastasis
Published on: March 14, 2021
3.2K
ROR1 and ROR2-novel targets for neuroblastoma
Hema Dave1, Donna Butcher2, Miriam Anver2
1Center for Cancer and Immunology Research, Children's National Health System, The George Washington University , Washington , DC , USA.
Pediatric Hematology and Oncology
|August 24, 2019
Summary
Receptor Tyrosine Kinase-Like Orphan Receptors 1 and 2 (ROR1 and ROR2) are promising targets for neuroblastoma (NBL) immunotherapy. Targeting ROR1 with antibodies enhanced natural killer cell cytotoxicity, suggesting a new treatment avenue for NBL relapse.
Area of Science:
- Immunology
- Oncology
- Molecular Biology
Background:
- Neuroblastoma (NBL) relapse is a major cause of mortality in high-risk patients despite advances in immunotherapy.
- Novel tumor-associated antigens are needed to improve immunotherapy efficacy and minimize toxicity.
- Receptor Tyrosine Kinase-Like Orphan Receptors 1 and 2 (ROR1 and ROR2) are expressed in various cancers with limited healthy tissue expression.
Purpose of the Study:
- To investigate ROR1 and ROR2 as potential immunotherapy targets in NBL.
- To evaluate the role of ROR1 and ROR2 in NBL migration and proliferation.
- To explore the efficacy of anti-ROR1 and anti-ROR2 immunotherapies for NBL.
Main Methods:
- Characterization of ROR1 and ROR2 mRNA and protein expression in NBL cell lines and patient samples.
- In vitro cytotoxicity assays using NK92 effector cells against NBL.
- Correlation analysis of ROR1/ROR2 expression with patient survival and prognosis.
Main Results:
- ROR1 and ROR2 are expressed across all stages of NBL.
- ROR1/ROR2 expression correlates with survival and prognosis in non-MYC amplified tumors.
- Anti-ROR1 antibody pretreatment enhanced NK92 cell-mediated cytotoxicity against NBL cells.
Conclusions:
- ROR1 and ROR2 represent novel targets for NBL immunotherapy.
- Combining anti-ROR1 antibodies with immune effectors like NK92 cells shows potential for off-the-shelf immunotherapy.
- Further research is warranted to explore combination therapies for ROR1-expressing malignancies.
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