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Updated: Jan 20, 2026

Calcium Phosphate Transfection of Primary Hippocampal Neurons
Published on: November 12, 2013
Optimization and Characterization of Calcium Phosphate Transfection in Mesenchymal Stem Cells
Chi-Wen Lo1, Tzuhua Lin1, Masaya Ueno1
1Department of Orthopaedic Surgery, Stanford University, Stanford, California.
Impact Statement:
Mesenchymal stem cells (MSCs) are a promising tool for cell therapy, and gene-modified MSCs further expand their applications. To take full advantage of MSCs as a therapeutic approach, developing effective gene transfer methods is critical. Calcium phosphate transfection is well-established and safe, but the protocols need to be optimized according to different cell types. Currently, there is no optimized protocol for MSCs. This study optimized the protocol of calcium phosphate transfection for MSCs and highlighted the importance of serum during the process of transfection. More interestingly, the behavior of gene overexpression in MSCs in the in vivo environment was verified.
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