Related Experiment Video
Updated: Jan 20, 2026

A Protocol for Real-time 3D Single Particle Tracking
Published on: January 3, 2018
Tracking Insulin Glycation in Real Time by Time-Resolved Emission Spectroscopy
Li Hung C Chung1, David J S Birch1, Vladislav Vyshemirsky2
1Photophysics Group, Centre for Molecular Nanometrology, Department of Physics, Scottish Universities Physics Alliance , University of Strathclyde , 107 Rottenrow East , Glasgow G4 0NG , U.K.
Abstract:
The application of time-resolved fluorescence sensing to the study of heterogenic biomolecular systems remains challenging because of the complexity of the resulting photophysics. Measuring the time-resolved emission spectroscopy (TRES) spectra can provide a more informative alternative to the modeling of the fluorescence decay that is currently employed. Here, we demonstrate this approach by monitoring real-time changes in intrinsic insulin fluorescence by TRES as a straightforward probe to directly measure kinetics of insulin aggregation and glycation. Our findings hold promise for monitoring the storage of insulin and its application in the control of diabetes and may support the development of more effective therapeutics against amyloidosis.
Related Concept Videos
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
Atomic Emission Spectroscopy: Lab
Atomic Emission Spectroscopy: Overview
Atomic Emission Spectroscopy: Instrumentation
Atomic Emission Spectroscopy: Interference
Molecular Spectroscopy: Absorption and Emission

