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Updated: Jan 20, 2026

Reverse Transcription Loop-Mediated Isothermal Amplification RT-LAMP Assay for the Specific and Rapid Detection of Tilapia Lake Virus
Published on: May 18, 2020
Development of a real-time loop-mediated isothermal amplification assay for detection of porcine circovirus 3
Huanan Wang1,2, Xiangnan Liu3,4, Fanwen Zeng3,4
1Department of Veterinary Medicine, Zhejiang Provincial Key Laboratory of Preventive Veterinary Medicine and College of Animal Sciences, Zhejiang University, Hangzhou, 310058, China.
Background:
Porcine circovirus type 3 (PCV3) is an emerging circovirus species, that has been reported in major pig-raising countries including the United States, China, South Korea, Brazil, Spain, and Poland.
Results:
A real-time loop-mediated isothermal amplification (LAMP) assay was developed for rapid detection of porcine circovirus 3 (PCV3). The method had a detection limit of 1 × 101 copies/μL with no cross-reactions with classical swine fever virus (CSFV) C strain, foot-and-mouth disease virus (FMDV), porcine circovirus 2 (PCV2) LG vaccine strain, porcine epidemic diarrhoea virus (PEDV), porcine respiratory and reproductive syndrome virus (PRRSV), or pseudorabies virus (PRV). The PCV3 positive detection rate of 203 clinical samples for the real-time LAMP assay was 89.66% (182/203).
Conclusions:
The real-time LAMP assay is highly sensitive, and specific for use in epidemiological investigations of PCV3.
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