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Supplemental Antibiotics in a Commercial Extender for Stallion Semen

Camilo Hernández-Avilés1, Charles C Love1, Rosanna Serafini1

  • 1Department of Large Animal Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX.

Journal of Equine Veterinary Science
|August 25, 2019
PubMed

Insights

Supplementing stallion semen extenders with amikacin disulfate and potassium penicillin G improved antimicrobial activity without harming sperm quality. This combination enhanced sperm motility and reduced bacterial growth during cooled storage.

Area of Science:

  • Animal Science
  • Reproductive Biology
  • Microbiology

Background:

  • Semen extenders commonly contain antibiotics to control bacterial contamination in stallion semen.
  • Evaluating the efficacy of different antibiotic combinations in semen extenders is crucial for maintaining sperm quality and viability.

Purpose of the Study:

  • To assess the impact of supplemental amikacin disulfate + potassium penicillin G (INRA-AP) or ticarcillin-clavulanate (INRA-TIM) in INRA 96 extender on stallion sperm function and antimicrobial activity.
  • To compare these treatments against an extender without supplemental antibiotics (INRA-C).

Main Methods:

  • Freshly extended semen was analyzed for sperm motion and plasma membrane integrity at 30 minutes.
  • Sperm quality parameters and bacterial colony-forming units (CFU/mL) were evaluated after 24 hours of cooled storage.
  • Antimicrobial activity was assessed by the proportion of culture plates with no bacterial growth.

Main Results:

  • No significant differences in sperm motility or membrane integrity were observed at 30 minutes across all groups.
  • After 24 hours of cooled storage, INRA-AP showed significantly higher progressive motility and straightness compared to INRA-C and INRA-TIM.
  • INRA-AP demonstrated superior antimicrobial activity, yielding lower CFU/mL and a higher percentage of sterile cultures.

Conclusions:

  • Supplementation of INRA 96 extender with amikacin disulfate + potassium penicillin G at the tested concentrations effectively controls bacterial growth.
  • This antibiotic combination improves stallion sperm quality during cooled storage without adverse effects.
  • INRA-AP represents a promising option for enhancing the antimicrobial properties of semen extenders.
Keywords:
AmikacinAntibioticPenicillinSemen extenderStallion sperm

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