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Scribble and Discs Large mediate tricellular junction formation.

Zohreh Sharifkhodaei1, Mary M Gilbert1, Vanessa J Auld2

  • 1Department of Zoology, University of British Columbia, Vancouver V6T 1Z4, Canada.

Development (Cambridge, England)
|August 25, 2019
PubMed
Summary
This summary is machine-generated.

Scribble (Scrib) and Discs Large (Dlg) proteins are essential for forming tricellular junctions (TCJs) in Drosophila epithelia. These proteins, along with Gliotactin and Bark Beetle, regulate TCJ integrity and localization.

Keywords:
Bark BeetleDiscs LargeDrosophilaGliotactinScribbleTricellular junction

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Area of Science:

  • Cell biology
  • Developmental biology
  • Epithelial biology

Background:

  • Epithelial integrity relies on junctional complexes that regulate cell adhesion and permeability.
  • Scribble (Scrib) and Discs Large (Dlg) are crucial scaffolding proteins involved in epithelial polarity and junction assembly.
  • Tricellular junctions (TCJs) are unique structures at the convergence of three cells, vital for epithelial function.

Purpose of the Study:

  • To investigate the role of Scribble (Scrib) and Discs Large (Dlg) in the formation and maintenance of tricellular junctions (TCJs) in Drosophila.
  • To identify interactions between Scrib/Dlg and other TCJ components like Gliotactin (Gli) and Bark Beetle (Bark).

Main Methods:

  • Utilized Drosophila melanogaster as a model organism.
  • Employed genetic analysis to study loss-of-function mutations in Scrib, Dlg, Gli, and Bark genes.
  • Investigated protein localization and complex formation using proximity and co-localization studies.

Main Results:

  • Scrib and Dlg are indispensable for the proper formation and localization of TCJs.
  • Scrib and Dlg interact with TCJ proteins Gliotactin and Bark Beetle, facilitating their recruitment to the junction.
  • Loss of Bark or Gli, or septate junction proteins Nrx-IV and Na+/K+ ATPase, leads to the mislocalization of the TCJ complex.
  • Specific domains of Scrib (PDZ1-2) and Dlg (GUK) are critical for the localization of Bark and Gli to the TCJ.

Conclusions:

  • Scribble and Discs Large are key components of the tricellular junction in Drosophila.
  • Scrib and Dlg form a functional complex with Gliotactin and Bark Beetle to ensure TCJ integrity.
  • This study elucidates a novel regulatory mechanism for TCJ formation and stability.