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Nucleic Acids

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Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
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Nucleic acid biosynthesis is a fundamental biochemical process that produces the purine and pyrimidine nucleotides essential for DNA and RNA synthesis. This pathway maintains a balanced nucleotide pool, preventing imbalances that could jeopardize genetic integrity and cellular function. Given the crucial role of nucleotides, their synthesis is tightly regulated to ensure proper cellular homeostasis.Purine BiosynthesisThe biosynthesis of purine nucleotides begins with ribose-5-phosphate, a...

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Related Experiment Video

Updated: Jan 20, 2026

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Kinetic Screening of Nuclease Activity using Nucleic Acid Probes

Published on: November 1, 2019

Multi-level patterning nucleic acid photolithography.

Kathrin Hölz¹, Erika Schaudy¹, Jory Lietard²

¹Institute of Inorganic Chemistry, Faculty of Chemistry, University of Vienna, Althanstrasse 14 (UZA II), 1090, Vienna, Austria.

Nature Communications

|August 25, 2019

Summary

This summary is machine-generated.

Researchers developed a photolithographic method for creating complex nucleic acid structures. This technique enables precise surface synthesis and modification, paving the way for advanced nanoscale devices and circuits.

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Area of Science:

Biotechnology
Nanotechnology
Materials Science

Background:

Nucleic acids offer versatile self-assembly for micro- and nanoscale devices.
Current fabrication methods require complex, multi-step processes for advanced constructs.

Purpose of the Study:

To develop a programmable, multi-level photolithographic approach for fabricating complex nucleic acid constructs.
To enable efficient in situ synthesis and post-synthetic modifications of nucleic acid structures.

Main Methods:

Utilized multi-level photolithography for large-scale in situ surface synthesis of nucleic acids.
Incorporated chemical and enzymatic modifications for post-synthetic processing.
Demonstrated micrometer-resolution patterning of nucleic acid structures.

Main Results:

Created high-complexity nucleic acid surface patterns with micrometer resolution.
Achieved linear and branched structures, multi-color fluorophore labeling.
Enabled programmable targeted oligonucleotide immobilization and cleavage.

Conclusions:

The developed photolithographic approach offers a versatile platform for fabricating complex nucleic acid nanostructures.
This method facilitates the integration of nucleic acid constructs into functional devices and circuits.
Provides a pathway for enhanced complexity and programmability in nucleic acid-based nanotechnology.