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Comparison of Methods for Isolating Entomopathogenic Fungi from Soil Samples
Published on: January 6, 2022
Comparison of Leptospira species isolated from environmental water and soil in Japan
Toshiyuki Masuzawa1, Rurika Uno1, Nana Matsuhashi1
1Laboratory of Microbiology and Immunology, Faculty of Pharmaceutical Sciences, Chiba Institute of Science (CIS), Choshi, Chiba, Japan.
Abstract:
Leptospira was isolated from environmental water in central Japan using selective medium comprising five antibiotics, namely sulfamethoxazole, trimethoprim, amphotericin B, fosfomycin, and 5-fluorouracil. Of 100 water samples 57 (57%) were culture-positive and 50 pure cultures were isolated. Of the 50 cultures isolated from water 48 were classified into a saprophytic clade on the basis of 16S ribosomal RNA gene sequences. However, it was previously reported that isolates from soil in Japan belonged to pathogenic, intermediate, and saprophytic clades, the current findings suggest less diversity of Leptospira species in environmental water than that in soil in Japan.
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