Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

7.2K
Transcriptional regulators bind to specific cis-regulatory sequences in the DNA to regulate gene transcription. These cis-regulatory sequences are very short, usually less than ten nucleotide pairs in length. The short length means that there is a high probability of the exact same sequence randomly occurring throughout the genome.  Since regulators can also bind to groups of similar sequences, this further increases the chances of random binding. Transcriptional regulators form...
7.2K
Cooperative Binding of Transcription Regulators02:13

Cooperative Binding of Transcription Regulators

2.5K
2.5K
Transcription Factors02:16

Transcription Factors

82.3K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.3K
Transcription Elongation Factors02:35

Transcription Elongation Factors

13.5K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
13.5K
Master Transcription Regulators02:23

Master Transcription Regulators

7.7K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.7K
From DNA to Protein03:06

From DNA to Protein

22.2K
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
22.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhanced stability of immobilized xylanase with nano‑zinc oxide/ethyl cellulose composite carrier for efficient xylan hydrolysis.

Food chemistry·2026
Same author

Distinct hydrolyzed Al-Ti species govern the coagulation behavior and subsequent anaerobic fermentation of CEPS sludge.

Water research·2026
Same author

The anion channel SLAH3 regulates flowering time in Arabidopsis thaliana.

Biochemical and biophysical research communications·2026
Same author

A bulk cell heterozygous knock-in strategy for targeted protein degradation.

bioRxiv : the preprint server for biology·2026
Same author

Development and Validation of a Nomogram for Predicting Sepsis Risk in Patients with Non-Ventilator Hospital-Acquired Pneumonia.

Biomedicines·2026
Same author

Dual-functional chitosan-hyaluronic acid dialdehyde nanoparticles for CD44-targeted Bcl-2 siRNA delivery and photothermal therapy in bladder cancer.

Journal of biotechnology·2026

Related Experiment Video

Updated: Jan 20, 2026

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
11:25

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

8.5K

Modulating DNA by polyamides to regulate transcription factor PU.1-DNA binding interactions.

Beibei Liu1, James K Bashkin2, Gregory M K Poon1

  • 1Department of Chemistry, Georgia State University, Atlanta, GA, 30303, USA.

Biochimie
|August 25, 2019
PubMed
Summary

Synthetic polyamides can regulate gene expression by targeting DNA. Researchers designed polyamides to control PU.1 DNA binding, finding most inhibit binding, while one molecule uniquely enhances it, offering new therapeutic design strategies.

Keywords:
ETS familyHairpin polyamidePU.1Transcription factor inhibitionβ-alanine

More Related Videos

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

9.2K
Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow
07:48

Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow

Published on: April 1, 2022

4.6K

Related Experiment Videos

Last Updated: Jan 20, 2026

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences
11:25

Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

Published on: February 11, 2019

8.5K
High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy
06:38

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

9.2K
Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow
07:48

Genome-wide Profiling of Transcription Factor-DNA Binding Interactions in Candida albicans: A Comprehensive CUT&RUN Method and Data Analysis Workflow

Published on: April 1, 2022

4.6K

Area of Science:

  • Molecular Biology
  • Medicinal Chemistry
  • Genetics

Background:

  • Hairpin polyamides are small molecules that bind DNA sequence-selectively.
  • Polyamides can alter DNA structure and influence protein-DNA interactions.
  • Aberrant gene expression, particularly of the transcription factor PU.1, is linked to acute myeloid leukemia (AML).

Purpose of the Study:

  • To design and synthesize novel hairpin polyamides.
  • To investigate the polyamides' ability to control the interaction between PU.1 and DNA.
  • To explore polyamides as potential therapeutic agents for diseases like AML.

Main Methods:

  • Design and synthesis of ten distinct hairpin polyamides.
  • Assessing the polyamides' capacity to disrupt PU.1-DNA binding.
  • Determining the binding affinities of the polyamides to DNA.

Main Results:

  • Nine out of ten synthesized polyamides effectively disrupted PU.1-DNA binding.
  • The inhibitory capacity of the polyamides correlated strongly with their DNA binding affinity.
  • One polyamide, FH1024, formed a ternary complex with PU.1 and DNA, acting as a potential weak agonist.

Conclusions:

  • Hairpin polyamides can be designed to modulate specific transcription factor-DNA interactions.
  • FH1024 represents a novel class of small molecules that can recruit proteins to DNA, suggesting new regulatory mechanisms.
  • These findings offer insights for developing polyamides as therapeutic agents targeting aberrant gene expression in diseases like AML.