Related Experiment Video
Updated: Jan 20, 2026

Identification of Fatty Acids in Bacillus cereus
Published on: December 5, 2016
Ectoine biosynthesis genes from the deep sea halophilic eubacteria, Bacillus clausii NIOT-DSB04: Its molecular and
Lawrance Anburajan1, Balakrishnan Meena1, Thonthula Sreelatha2
1Atal Centre for Ocean Science and Technology for Islands, National Institute of Ocean Technology, Port Blair, 744 103, Andaman and Nicobar Islands, India.
Abstract:
Ectoine, the most prominent osmolyte in nature, is a vital compatible solute present in halophilic bacterium. It protects the cellular biomolecules of the halophilic bacteria and retains their intrinsic function from extreme circumstances. In the current research, ectoine biosynthesis gene cluster (ectABC) in Bacillus clausii NIOT-DSB04 was expressed heterologically in E. coli M15 (pREP4). RP-HPLC resolved several fractions of the purified recombinant product, one of which had been confirmed as ectoine. The recombinant ectoine was further characterized by 1H and 13C Nuclear Magnetic Resonance. The purified recombinant ectoine was also authenticated by FT-IR studies with the existence of ester carbonyl and C-H group. In IPTG induced E. coli M15 transgenic cells, the enzymatic activity of the ectA, B and C genes were found to be higher than that of uninduced cells.
Related Concept Videos
Biosynthesis in Bacteria
Biosynthesis of Polysaccharides
Biosynthesis of Lipids
Biosynthesis of Nucleic Acids
Insulin: Biosynthesis, Chemistry, and Preparation
Damage or functional impairment of β-cells inhibits insulin production, leading to diabetes. Diabetes treatment...
Effect of Sea Water on Concrete
Concrete in areas between tide marks,...

