Nanomaterials meet zebrafish: Toxicity evaluation and drug delivery applications

With the rapid development of engineered nanomaterials for various applications, in vivo toxicological studies for evaluating the potential hazardous effects of nanomaterials on environmental and human safety are in urgent need. Zebrafish has long been considered as the "gold standard" for biosafety assessments of chemicals and pollutants due to its high fecundity, cost-effectiveness, well-characterized developmental stages, optical transparency, and so forth. Thus, zebrafish holds great potential for high-throughput nanotoxicity screening. In this review, we summarize the in vivo toxicological profiles of different nanomaterials, including Ag nanoparticles (NPs), CuO NPs, silica NPs, polymeric NPs, quantum dots, nanoscale metal-organic frameworks, etc, in zebrafish and focus on how the physicochemical properties (e.g., size, surface charge, and surface chemistry) of these nanomaterials influence their biosafety. In addition, we also report the recent advances of the in vivo delivery of nanopharmaceuticals using zebrafish as the model organism for therapeutic assessment, biodistribution tracking, and the controlled release of loaded drugs. Limitations and special considerations of zebrafish model are also discussed. Overall, zebrafish is expected to serve as a high-throughput screening platform for nanotoxicity and drug delivery assessment, which may instruct the design of safe nanomaterials and more effective nanomedicines.