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Updated: Jan 20, 2026

Mass Cytometry: Protocol for Daily Tuning and Running Cell Samples on a CyTOF Mass Cytometer
Published on: November 2, 2012
Application value of CyTOF 2 mass cytometer technology at single-cell level in human gastric cancer cells
Shuangshuang Han1, Xia Jiang2, Xiao-Feng Sun3
1Departments of Endoscopy Center, The First Hospital of Hebei Medical University, 050031, Shijiazhuang, Hebei, China.
Abstract:
Chemotherapy and radiotherapy are main adjuvant therapies for the treatment of gastric cancer, the treatment effects are individual difference, but the specific mechanism is unknown. CyTOF 2 mass cytometer (CyTOF) enables the detecting up to 135 parameters on single cell, the emergence of which is an opportunity for proteomics research. We first tried to apply CyTOF technique to gastric cancer cells. We verified applicability of CyTOF in gastric cancer cells, and analyzed the responses of seventeen proteins to chemoradiotherapy in human gastric cancer AGS cells. To analyze the high dimensional CyTOF data, we used two statistical and visualization tools including viSNE and Citrus. Two specific clusters were found which had differences in protein expression profiles. CyTOF technology is proved feasibility and value at single cell level of gastric cancer.
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