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Updated: Jan 20, 2026

Shock Wave Application to Cell Cultures
Published on: April 8, 2014
Extracorporeal Shock Wave Stimulates Angiogenesis and Collagen Production in Facial Soft Tissue
Abdulmonem Alshihri1, Peer W Kämmerer2, Diana Heimes3
1College of Dentistry, King Saud University, Riyadh, Kingdom of Saudi Arabia; Harvard School of Dental Medicine, Boston, Massachusetts; VA Boston Healthcare System, Boston, Massachusetts.
Background:
This study investigated the efficacy of extracorporeal shock wave (ESW) application in stimulating dermal thickness, vascularity, and collagen synthesis of facial skin in a large animal model.
Materials And Methods:
The facial skin of the maxillary and mandibular areas of goats (n = 6 per group) was treated with ESWs of different intensities (0.15 and 0.45 mJ/mm2; 1000 pulses). After 4 d, histology and immunohistochemistry were used to evaluate the following: dermal thickness, total number and abundance of microvessels, amount of type 1 collagen, and α-smooth muscle actin expression.
Results:
Dermal thickness, number and abundance of microvessels, and collagen synthesis increased after ESW application at both intensities (each P < 0.05). When comparing ESW groups, the highest collagen abundance was seen after 0.15 mJ/mm2 (P = 0.034), whereas the highest number of microvessels was detected after treatment with 0.45 mJ/mm2 (P = 0.002).
Conclusions:
A single-session application of focused low-energy ESWs to facial skin can increase dermal thickness by stimulating collagen production and local microcirculation. These findings commend the technique for future investigation for pretreatment of local or microvascular skin flaps to enhance tissue healing.
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