Related Experiment Videos
Cell surface oligosaccharide modulation during differentiation: IV. Normal and transformed cell growth control
P L Mann1, C M Swartz, D T Holmes
1Department of Anatomy, University of New Mexico, School of Medicine, Albuquerque 87131.
Mechanisms of Ageing and Development
|July 1, 1988
Summary
Cell surface oligosaccharides regulate cell growth. Changes in mannose and galactose binding correlate with cell senescence and cancer, suggesting a key role in growth control.
Area of Science:
- Cell Biology
- Glycobiology
- Cancer Research
Background:
- Cell surface oligosaccharide affinity and binding decrease as normal cells age (senescence).
- Understanding these changes is crucial for comprehending growth regulation in normal and cancerous cells.
Purpose of the Study:
- To compare cell surface oligosaccharide regulation in senescence, growth inhibition, and neoplastic growth.
- To investigate the role of cell surface oligosaccharides in controlling cell growth.
Main Methods:
- Utilized sensitive, amplified lectin/specific-ligand competition analysis.
- Compared non-senescent and senescent human fibroblasts (IMR-90) with canine glioma cells (CG cells).
- Investigated the effects of alpha-mannosidase II inhibitor, Ricinus communis lectin, and pokeweed mitogen on CG cells.
Main Results:
- Non-senescent IMR-90 cells down-regulated mannosyl and galactosyl specificities with growth inhibition.
- Senescent IMR-90 cells showed differential regulation of mannosyl and galactosyl residues under contact inhibition.
- CG cells lacked density-dependent regulation; modulation altered their phenotype and oligosaccharide expression.
Conclusions:
- Cell surface oligosaccharides play a controller function in cell growth.
- Changes in oligosaccharide expression are linked to distinct growth regulation states, including senescence and neoplastic transformation.