Related Experiment Video

Updated: Jan 20, 2026

An Effective Mouse Model of Unilateral Renal Ischemia-Reperfusion Injury
05:53

An Effective Mouse Model of Unilateral Renal Ischemia-Reperfusion Injury

Published on: July 15, 2021

8.1K

The protective effect of cannabinoid type 2 receptor activation on renal ischemia-reperfusion injury

Murat Çakır1, Suat Tekin2, Züleyha Doğanyiğit3

  • 1Faculty of Medicine, Department of Physiology, University of Yozgat Bozok, Yozgat, 66200, Turkey. murat.cakir@bozok.edu.tr.

Molecular and Cellular Biochemistry
|August 26, 2019
PubMed

Insights

Activation of the Cannabinoid type 2 (CB2) receptor with JWH-133 demonstrated a protective effect against kidney ischemia reperfusion (IR) injury. This therapeutic approach reduced inflammation and apoptosis, suggesting potential for treating acute renal failure.

Area of Science:

  • Nephrology
  • Immunology
  • Pharmacology

Background:

  • Kidney ischemia reperfusion (IR) injury is a significant cause of acute renal failure, characterized by inflammatory and apoptotic processes.
  • The Cannabinoid type 2 (CB2) receptor is implicated in modulating inflammatory and apoptotic pathways and is present in renal glomeruli and tubules.

Purpose of the Study:

  • To investigate the protective effects of selective CB2 receptor activation using JWH-133 against renal IR injury in a rat model.
  • To evaluate the impact of JWH-133 on histological damage, inflammatory markers, and apoptotic indicators in the kidneys.

Main Methods:

  • Male Sprague-Dawley rats underwent 45 minutes of bilateral ischemia followed by 24 hours of reperfusion.
  • Three different doses of JWH-133 (0.2, 1, and 5 mg/kg) were administered at the onset of ischemia.
  • Histological examination and immunohistochemical analysis were performed on renal tissue, alongside serum biochemical marker analysis.

Main Results:

  • JWH-133 administration at all tested doses significantly reduced glomerular and tubular damage.
  • Immunohistochemical analysis revealed decreased levels of nuclear factor-κB, tumor necrosis factor-alpha, interleukin-1beta, and caspase-3 in renal tissue.
  • Serum levels of various biomarkers, including tumor necrosis factor-alpha, blood urea nitrogen, creatinine, kidney injury molecule-1, neutrophil gelatinase-associated lipocalin, Cystatin C, and interleukins, were reduced by JWH-133 treatment.

Conclusions:

  • CB2 receptor activation with JWH-133 exhibits a curative effect against kidney IR injury.
  • JWH-133 demonstrates potential as a novel therapeutic agent for preventing and treating kidney IR damage.
Keywords:
Cannabinoid type 2 receptorIschemia–reperfusion injuryJWH-133Kidney

More Related Videos

Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research03:13

Technical Refinement of a Bilateral Renal Ischemia-Reperfusion Mouse Model for Acute Kidney Injury Research

Published on: November 3, 2023

2.9K
Renal Ischaemia Reperfusion Injury: A Mouse Model of Injury and Regeneration12:27

Renal Ischaemia Reperfusion Injury: A Mouse Model of Injury and Regeneration

Published on: June 7, 2014

51.2K

Related Concept Videos

Types of Receptors: Internal Receptors01:07

Types of Receptors: Internal Receptors

Many cellular signals are hydrophilic and cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind intracellular receptors that reside within the cell cytoplasm or nucleus. Many mammalian steroid hormones and nitric oxide (NO) gas use this cell signaling mechanism.
Similar to membrane-bound receptors, the binding of a ligand to the intracellular receptor of causes a conformational change in the...
31.6K
Types of Receptors: Cell Surface Receptors01:28

Types of Receptors: Cell Surface Receptors

Cell-surface receptors, also known as transmembrane receptors, are cell surface, membrane-anchored (integral) proteins that bind to external ligand molecules. This type of receptor spans the plasma membrane and performs signal transduction, converting an extracellular signal into an intracellular signal. Ligands that interact with cell-surface receptors do not have to enter the cell that they affect. Cell-surface receptors are also called cell-specific proteins or markers because they are...
26.8K
Diabetes Mellitus: Type 2 and Gestational01:22

Diabetes Mellitus: Type 2 and Gestational

Type 2 diabetes, characterized by insulin resistance, arises when the insulin receptors on cells lose responsiveness to insulin, diminishing the cell's capacity to take up glucose, resulting in elevated blood glucose levels. To receive a diagnosis of Type 2 diabetes, a series of blood glucose tests are necessary to assess whether the blood glucose falls within normal parameters. If the result is out of the normal range, a patient may be diagnosed as prediabetic or diabetic, depending on the...
4.4K
CNS Stimulants: Cocaine, Amphetamines and Cannabinoids01:24

CNS Stimulants: Cocaine, Amphetamines and Cannabinoids

CNS stimulants, such as cocaine, amphetamines, and cannabinoids, have varying structures and mechanisms of action that lead to different therapeutic effects and side effects. Cocaine, with its molecular formula C17H21NO4, is a tropane alkaloid and a tertiary amino compound. It has two chemical forms: the hydrochloride salt and the "freebase." The former is in powder form, while the latter involves removing the hydrochloride salt to create a form that can be smoked. Cocaine exerts its...
801
Chemotherapy-Induced Nausea and Vomiting: Cannabinoids01:21

Chemotherapy-Induced Nausea and Vomiting: Cannabinoids

Tetrahydrocannabinol (THC) is a phytocannabinoid that primarily interacts with the CB1 receptor, a type of G protein-coupled receptor (GPCR) predominantly in and around the chemoreceptor trigger zone (CTZ) and emetic center. THC also blocks the serotonin receptor activity in the dorsal vagal complex (DVC) by inhibiting serotonin release. THC exerts its anti-emetic effects through these interactions, which are beneficial for patients undergoing chemotherapy.
Two synthetic agonists of THC,...
678
Internal Receptors01:31

Internal Receptors

Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
74.3K