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Niazirin from Moringa oleifera Lam. attenuates high glucose-induced oxidative stress through PKCζ/Nox4 pathway

Fang Wang1, Yifan Bao1, Xinchun Shen1

  • 1College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, China.

Phytomedicine : International Journal of Phytotherapy and Phytopharmacology
|August 27, 2019
PubMed
Abstract

Insights

Niazirin from Moringa oleifera demonstrates significant antioxidant activity, reducing reactive oxygen species (ROS) and vascular smooth muscle cell proliferation. This natural compound shows promise in preventing diabetic atherosclerosis.

Area of Science:

  • Cardiovascular Pharmacology
  • Natural Product Chemistry
  • Oxidative Stress Research

Background:

  • Diabetic atherosclerosis is linked to hyperglycemia-induced reactive oxygen species (ROS), which promote vascular smooth muscle cell (VSMC) proliferation.
  • Moringa oleifera extracts possess antioxidant properties, but specific compounds' cardiovascular effects are understudied.

Purpose of the Study:

  • To investigate the antioxidant effects of niazirin, a compound from Moringa oleifera, under hyperglycemic conditions.

Main Methods:

  • In vitro antioxidant assays (FRAP, ABTS, DPPH) and cell viability tests on high glucose-induced VSMCs.
  • Measurement of ROS production, malondialdehyde (MDA), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), and glutathione peroxidase (GPx).
  • Western blot analysis of protein kinase C zeta (PKCζ) and NADPH oxidase 4 (Nox 4) expression in vitro and in vivo.

Main Results:

  • Niazirin exhibited potent free radical scavenging activity and attenuated VSMC proliferation under high glucose.
  • It reduced ROS and MDA levels while increasing T-AOC, SOD, and GPx in hyperglycemic VSMCs and mice.
  • Niazirin inhibited high glucose-induced PKCζ activation and Nox4 protein expression.

Conclusions:

  • Niazirin from Moringa oleifera possesses significant antioxidant properties.
  • It effectively mitigates oxidative stress and cellular damage associated with hyperglycemia.
  • Niazirin is a potential natural antioxidant for preventing diabetic atherosclerosis.
Keywords:
Diabetic atherosclerosisMoringa oleiferaNatural antioxidantNiazirinReactive oxygen species

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