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Related Experiment Videos

Catecholamine binding and concentrations in acute phase plasma after surgery.

G Sager1, T Trovik, L Slørdal

  • 1Department of Pharmacology, University of Tromsø, Norway.

Scandinavian Journal of Clinical and Laboratory Investigation
|September 1, 1988
PubMed
Summary

Biologically active catecholamine levels in human plasma are primarily influenced by total concentrations, not protein binding. This study found that while protein binding of noradrenaline and adrenalin increased post-surgery, total concentrations drove variability.

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Area of Science:

  • Pharmacology and Toxicology
  • Clinical Chemistry
  • Biochemistry

Background:

  • Catecholamines, including noradrenaline (NA) and adrenalin (Adr), are crucial for physiological responses.
  • Plasma protein binding can influence the concentration of free, biologically active hormones.
  • Surgical trauma can induce an acute phase reaction, altering plasma protein concentrations like albumin (HSA) and alpha-1 acid glycoprotein (AAG).

Purpose of the Study:

  • To determine if bound fractions or total concentrations of catecholamines dictate the variability of their biologically active levels in human plasma.
  • To investigate changes in catecholamine binding and concentrations following major hip surgery.

Main Methods:

  • Measurement of bound and total concentrations of noradrenaline (NA) and adrenalin (Adr) in plasma from five subjects before and after major hip surgery.

Related Experiment Videos

  • Analysis of plasma concentrations of albumin (HSA) and alpha-1 acid glycoprotein (AAG) to assess the acute phase reaction.
  • Comparison of catecholamine binding percentages and total concentrations to identify factors influencing biologically active levels.

    • Main Results:

    • Pre-surgery binding was 23.0% for NA and 18.4% for Adr.
    • Post-surgery, catecholamine binding increased to 30.9% for NA and 24.0% for Adr by day five.
    • Surgical trauma led to decreased HSA and increased AAG concentrations, indicating an acute phase reaction.
    • Considerable inter- and intraindividual variability in total catecholamine concentrations was observed.
    • The variability in biologically active catecholamine concentrations was found to be primarily dependent on total plasma concentrations, not protein binding.

    Conclusions:

    • The variability of biologically active catecholamine concentrations in human plasma after major hip surgery is mainly determined by total plasma concentrations.
    • Plasma protein binding of catecholamines plays a lesser role in the observed variability of their active concentrations in this context.
    • Understanding these dynamics is crucial for interpreting catecholamine levels in clinical settings, particularly following surgical stress.