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[Interaction of Neisseria meningitidis with eukaryotic cells in a mixed infection in vitro]
Abstract:
To study the adhesion of meningococci under the conditions of a monoinfection and mixed infection (in association with influenza virus), the experimental model of mixed influenzal and meningococcal infection has been created in the culture of epithelial cells HEp-2. On this model in increase in the intensity of the adhesion of meningococci to eukaryotic cells, as well as in the intensity of the meningococcal colonization of such cells, after their preliminary infection with influenza virus has been observed. The study has revealed that in mixed infection the adsorption of extracellular virions onto the surface of bacteria occurs. During this adsorption viral processes directly interact with the microcapsule of the meningococcus.
Insights
Influenza virus infection enhances meningococcal adhesion and colonization of epithelial cells. This occurs as influenza virus particles attach to meningococci, interacting with their microcapsule during mixed infections.
Area of Science:
- Microbiology
- Virology
- Cell Biology
Background:
- Meningococci (Neisseria meningitidis) are significant human pathogens.
- Understanding pathogen interactions is crucial for disease control.
- Influenza virus is a common respiratory pathogen.
Purpose of the Study:
- To investigate meningococcal adhesion and colonization in a mixed infection model.
- To determine the effect of influenza virus on meningococcal interactions with host cells.
Main Methods:
- Established an experimental model of mixed influenza and meningococcal infection using HEp-2 epithelial cells.
- Observed and quantified meningococcal adhesion and colonization intensities.
Main Results:
- Preliminary infection of HEp-2 cells with influenza virus significantly increased meningococcal adhesion.
- Influenza virus preliminary infection also enhanced meningococcal colonization of HEp-2 cells.
- Extracellular influenza virions were observed to adsorb onto the surface of meningococci, interacting with the bacterial microcapsule.
Conclusions:
- Influenza virus coinfection promotes meningococcal adhesion and colonization of epithelial cells.
- Viral adsorption to meningococci is a key mechanism in this enhanced interaction.
- These findings highlight the complex interplay between viral and bacterial pathogens.