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Xenobiotic oxidation by cytochrome P-450-enriched extracts of Streptomyces griseus
M K Trower1, F S Sariaslani, F G Kitson
1E.I. Du Pont de Nemours & Co. (Inc.), Central Research and Development Department, Wilmington, DE 19880-0228.
Biochemical and Biophysical Research Communications
|December 30, 1988
Abstract:
Crude extracts of Streptomyces griseus grown on soybean flour-enriched medium contain high levels of cytochrome P-450. The cytochrome P-450-enriched fractions, obtained by ammonium sulfate fractionation (30-50% saturation), catalyze the NADPH-dependent oxidation of a variety of xenobiotics when complemented with both spinach ferredoxin:NADP+ oxidoreductase and spinach ferredoxin. Reactions observed are aromatic, benzylic and alicyclic hydroxylations, O-dealkylation, non-aromatic double bond epoxidation, N-oxidation and N-acetylation.