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Updated: Jan 20, 2026

Preparation, Purification, and Use of Fatty Acid-containing Liposomes
Published on: February 9, 2018
Study of release kinetics and degradation thermodynamics of ferric citrate liposomes
Shan Wang1, Wenxin Li1, Kaiyue Sun1
1College of Chemistry and Material Science, Hebei Normal University, Shijiazhuang, 050024, China.
Abstract:
Ferric citrate liposome (FAC-Lip) with good sustained-released property was prepared by the rotary-evaporated film-ultrasonic method, and characterized by TEM, DLS, zeta potential and encapsulation efficiency (EE%). The effects of membrane material ratios (mPC: mchol = 8:1, 10:1 and 12:1) and drug lipid ratios (mFAC: mPC = 1:4, 1:6.5 and 1:8) on the release of FAC-Lip were examined. The in vitro release kinetic models and mechanisms of FAC-Lip in artificial gastric juice (SGF) and artificial intestinal juice (SIF) compared with free-FAC were determined. The thermal degradation in PBS was also determined. The results showed that FAC-Lip with membrane material ratio (10:1) and drug lipid ratio (1:6.5) had the optimal sustained-released property, unilamellar vesicles with uniform size (178 ± 2.12 nm), negative charge (-56 ± 3.51 mV) and high encapsulation efficiency (72.77 ± 0.42%). The in vitro release kinetic models of FAC-Lip were two-phase kinetics model and the release mechanisms were non-Fick diffusion both in SGF and SIF. The thermal degradation of FAC-Lip was an endothermic and spontaneous reaction. The results may be helpful in optimizing drug-liposome design, application in food and medicine industries, and furthermore, predicting and guiding medication in vivo.
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