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Analysis of Histone Antibody Specificity with Peptide Microarrays
Published on: August 1, 2017
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Protein domain microarrays as a platform to decipher signaling pathways and the histone code
Jianji Chen1, Cari Sagum2, Mark T Bedford2
1Department of Epigenetics and Molecular Carcinogenesis, The University of Texas MD Anderson Cancer Center, Smithville, TX 78957, USA; Graduate Program in Genetics & Epigenetics, The University of Texas MD Anderson Cancer Center UTHealth Graduate School of Biomedical Sciences, Houston, TX 77030, USA.
Methods (San Diego, Calif.)
|August 27, 2019
Summary
Protein domain microarrays rapidly identify protein interactions and potential drug targets. This review details their history, applications, and future directions in biomedical research.
Area of Science:
- Biochemistry and Molecular Biology
- Proteomics
- Biomedical Research
Background:
- Signal transduction relies on protein interactions modulated by posttranslational modifications (PTMs).
- Protein domains act as crucial readers of PTM signals.
- Protein domain microarrays offer a high-throughput method for studying these interactions.
Purpose of the Study:
- To review the history, development, and applications of protein domain microarrays.
- To highlight methods used at the Protein Array and Analysis Core (PAAC).
- To discuss the identification of novel protein-protein interactions and blocking small molecules.
Main Methods:
- Utilizing protein domain microarrays for high-throughput screening.
- Focusing on techniques employed by the PAAC.
- Investigating the identification of protein-protein interactions and small molecule inhibitors.
Main Results:
- Protein domain microarrays are effective for identifying protein-protein interactions.
- This technology can discover small molecules that disrupt these interactions.
- The PAAC employs specific approaches for array development and analysis.
Conclusions:
- Protein domain microarrays are a valuable tool in biomedical research.
- The technique facilitates the discovery of novel interactions and therapeutic agents.
- Addressing technical limitations and future advancements is essential for the field.
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